摘要
本实验对苦丁茶叶制虫茶粗多酚(KMICP)的抗氧化、抗突变效果和对人肝癌SMMC-7721细胞体外抗癌效果进行了观察。25、50、75和100μg/mL浓度KMICP处理下,对DPPH自由基的清除能力分别为27.8%、46.6%、85.9%和99.8%。1.25和2.5mg/皿浓度的KMICP对MNNG(N-甲基-N'-硝基-亚硝基胍)诱发突变表现出的49.5%和78.1%的抗突变能力,对AFB1(黄曲霉毒素B1)诱发突变的抑制效果也达到54.8%和76.4%。KMICP对体外生长的SMMC-7721细胞也表现出了明显的抑制作用,浓度达到150μg/mL后,癌细胞的生长被完全抑制。通过RT-PCR检验可以观察到KMICP处理SMMC-7721细胞后,癌细胞的Bax、IκB-α、TIMP-1和TIMP-2表达上升,Bcl-2和NF-κB表达下降,且高浓度的KMICP较低浓度对基因表达的影响更为明显。这些实验结果充分的说明了KMICP具有很好的体外抗氧化、抗突变和抗癌效果。
In this study, the antioxidant, antimutagenic and in vitro anticancer effects of crude polyphenols in insect tea made from Kuding tea leaves（KMICP） onhumanhepatoma cells SMMC-7721 were investigated. The DPPH radical scavenging activity after treatment using 25, 50, 75, and 100 μg/m L KMICP was 27.8%, 46.6%, 85.9%, and 99.8%, respectively. KMICP at concentrations of 1.25 and 2.5mg/plate showed antimutagenic effects on N-methyl-N＇-nitro-N-nitrosoguanidine（MNNG）-induced mutation（49.5% and 78.1%, respectively） and aflatoxin B1（AFB1）-induced mutation（54.8% and 76.4%, respectively）. KMICP also showed significant inhibitory activity on SMMC-7721 cells in vitro, while the growth of cancer cells was completely inhibited at 150 μg/m L concentration. By reverse-transcription polymerase chain reaction（RT-PCR） conducted on KMICP-treated SMMC-7721 cells, it was found that Bax, IκB-α, TIMP-1, and TIMP-2 gene expression in cancer cells was increased, Bcl-2 and NF-κB gene expression was decreased, and the effect ofhigh-concentration KMICP was more significant than that of low-concentration KMICP. These results demonstrate that KMICP shows good in vitro antioxidant, antimutagenic, and anticancer properties.
出处
《现代食品科技》
EI
CAS
北大核心
2015年第3期24-28,17,共6页
Modern Food Science and Technology
基金
重庆市教委科学技术研究项目(KJ1401402)
重庆第二师范学院引进高层次人才科研启动费
重庆第二师范学院创新团队计划(KYC-cxtd03-20141002)
关键词
虫茶
苦丁茶
多酚
抗突变
抗癌
insect tea
Kuding tea
polyphenol
antimutagenicity
anticancer
作者简介
赵欣（1981-），男，博士，教授，研究方向为食品营养和功能性食品
通讯作者：王强（1982-），男，副教授，研究方向为食品营养和功能性食品
