摘要
目的 探讨精囊凝胶蛋白Ⅰ （SgⅠ）与慢性复发性精囊炎的关系,分析慢性精囊炎发生发展的分子机制.方法 采用反转录-聚合酶链反应（RT-PCR）、免疫组织化学及Western blot技术分别检测10例正常精囊组织及28例慢性复发性精囊炎组织中Sg Ⅰ mRNA、蛋白表达差异.结果 RT-PCR结果显示,8例精囊炎组织中Sg Ⅰ mRNA水平显著低于正常精囊组织,免疫组织化学法及Western blot结果示分别有83.3％ （10/12）及75.0％（6/8）的精囊炎组织中Sg Ⅰ蛋白为低表达.结论 SgⅠ低表达在慢性精囊炎的发生发展中起重要作用.
Objective To investigate the relationship between semenogelin Ⅰ （Sg Ⅰ） expression and seminal vesiculitis,and the possible molecular mechanism of seminal vesiculitis.Methods Reverse transcription-polymerase chain reaction （RT-PCR）,immunohistochemistry and Western blotting were respectively performed to explore Sg Ⅰ mRNA and protein expression differences between 10 cases of normal seminal vesicle and 28 cases of chronic seminal vesiclitis.Results RT-PCR showed that Sg Ⅰ mRNA level in 8 cases of seminal vesiculitis was significantly lower than that in control group.Immunohistochemistry and Western blotting resulted in 83.3% （10/12） and 75.0% （6/8） of low Sg Ⅰ expression in seminal vesiculitis samples.Conclusion The low expression of Sg Ⅰ plays a vital role during the development of seminal vesilculitis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第3期647-649,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81270685）
关键词
精囊炎
精囊凝胶蛋白Ⅰ
精囊镜
抗菌活性
Seminal vesiculitis
Semenogelin Ⅰ
Seminal vesiculoscopy
Antibacterial ac-tivity
作者简介
通信作者：王增军，Email：zeng,junwang@njmu．edu．cn
