摘要
目的 观察去甲斑蝥素对人胆管癌RBE细胞组织化学及超微结构的影响,探讨去甲斑蝥素对胆管癌细胞作用机制.方法 体外培养胆管癌RBE细胞,采用不同5-40 mg/L去甲斑蝥素处理,吖啶橙/溴化乙锭（AO/EB）染色统计RBE细胞凋亡率,并观察形态学变化,扫描电镜观察去甲斑蝥素影响下胆管癌RBE细胞表面超微结构的变化,透射电镜观察细胞内超微结构的变化.结果 应用AO/EB荧光染色观察,随去甲斑蝥素剂量加大,胆管癌RBE细胞凋亡率升高,40 mg/L作用24 h后凋亡细胞占（83.1±15.6）％.光镜和扫描电镜下形态学呈现典型的凋亡变化,可见凋亡小体的形成.透射电镜可以观察到细胞核呈新月状改变.结论 去甲斑蝥素可以引起胆管癌RBE细胞显微和超微结构改变,这些改变可能与细胞凋亡和侵袭力改变等变化密切相关.
Objective To observe the effect of norcantharidin on cytohistochemistry and ultrastructure of human cholangiocarcinoma RBE cells,and investigate the action mechanism of norcantharidin inducing apoptosis of RBE cells.Methods The RBE cells were cultured in vitro,followed by treatment with various concentrations of norcantharidin for 24 h.Acridine orange/ethidium bromide （AO/EB） staining was used to determine the inhibition rate and morphological changes.The ultrastructural changes were studied by scanning electron microscopy and transmission electron microscopy.Results AO/EB staining showed that with increasing dose of norcantharidin,apoptosis rate of RBE cells was increased.After treatment with 40 mg/L norcantharidin for 24 h,the apoptotic cells accounted for （83.1 ± 15.6）%.Light microscopy and scanning electron microscopy showed typical apoptotic changes,and the formation of apoptotic bodies.The nucleus crescent-like change also could be observed by transmission electron microscopy.Conclusion Norcantharidin induces the morphological and ultrastructural changes in RBE cells,which have close relationship with apoptosis and invasion.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第3期519-521,共3页
Chinese Journal of Experimental Surgery
关键词
胆管癌
去甲斑蝥素
形态学
Cholangiocarcinoma
Norcantharidin
Morphology
作者简介
通信作者：刘三光，Email：sanguang1998@sina．com
