摘要
基于已建立的蒙古沙冬青[Ammopiptanthus mongolicus(Masxim.)Cheng f.]根的转录本数据库,分离到1个编码DREB类转录因子基因,命名为AmDREB2.1。该序列全长978 bp,包括531 bp的开放阅读框(ORF),编码176个氨基酸,具有典型的DREB转录因子保守的AP2结构域。实时荧光定量PCR分析表明,该基因能在根、叶中表达,但对干旱、低温响应不同,AmDREB2.1主要参与根的干旱胁迫应答。
A new dehydration responsive element binding protein(DREB)transcription factor gene, which was named as AmDREB2.1, was isolated from Ammopiptanthus mongolicus(Masxim.)(Cheng f.)root transcriptome database, that it had been established in our previous work. The full length of the AmDREB2.1 cDNA was 978 bp, including a single 531 bp opening reading frame which encoded a 176-amino acid peptide with a conserved AP2 domain. Quantitative real-time PCR analysis revealed that the Am DREB2.1 expressed in leaf and root of A. mongolicus, but there were different expression patterns under drought or low temperature stress respectively, and it could be mainly induced by drought in root.
出处
《生物技术通报》
CAS
CSCD
北大核心
2015年第3期108-114,共7页
Biotechnology Bulletin
基金
国家自然科学基金项目(31370356)
中央民族大学一流大学一流学科建设项目(YLDX01013)
国家大学生创新训练项目(GCCX2013110015)
中央民族大学研究生科研创新项目(K2014044)
作者简介
李章磊,男,硕士,研究方向:植物分子生物学;E-mail:lizhanglei2008@126.com
通讯作者:周宜君,女,博士,教授,研究方向:植物分子生物学;E-mail:queenzhou@263.net