摘要
目的:研究蒙药匝迪阿瓦治疗应激性心肌损伤的作用机制。方法:将Wistar大白鼠随机分成三组:正常对照组、制动冰泳组和匝迪治疗组,分别用HE染色法光镜观察心肌组织病理变化、ELISA法检测血清中肌钙蛋白I(c Tn I)、肿瘤坏死因a(TNF-a)、白细胞介素6(IL-6)的含量、用TUNEL法检测心肌细胞凋亡。结果:制动冰泳组大鼠心肌损伤明显,心肌细胞凋亡显著,血清中c Tn I、TNF-a、IL-6的含量增加(P<0.01);匝迪治疗组心肌损伤较轻,心肌细胞凋亡较少,血清中c Tn I、TNF-a、IL-6的含量减少(P<0.01)。结论:蒙药匝迪阿瓦可能通过抑制TNF-a、IL-6的释放,减轻炎症反应,减少心肌细胞凋亡,从而减轻大鼠心肌损伤。
Objective： To study the effective mechanism of the Mongolian nutmeg on stress-induced myocardial injury. Methods Wistar rats were randomly divided into three groups： control group,braking and ice swimming group and nutmeg treatment group. Pathological changes of myocardium were observed with the HE staining under the light microscopy. Troponin I（ c Tn I）,tumor necrosis factor a（ TNF-a） and interleukin-6（ IL- 6） levels were detected by ELISA in serum. Cardiac myocyte apoptosis was detected by TUNEL assay. Results Compared with normal control group,myocardial injury and cardiomyocyte apoptosis are both increased significantly in braking and ice swimming group,and the contents of c Tn I,TNF- a,IL- 6 are also increased in Serum（ P〈0. 01）. However,Compared with braking and ice swimming group,myocardial injury and cardiomyocyte apoptosis are less in nutmeg treatment group,and the contents of c Tn I,TNF-a,IL-6 are also decreased in Serum（ P〈0. 01）. Conclusion stress-induced myocardial injury may be related with myocardial apoptosis mediated by the inflammatory mediators tumor necrosis factor-a,IL-6. Moreover,Mongolian nutmeg had protective effects on stress-induced myocardial injury by reducing tumor necrosis factor-a,IL-6,and inhibiting cardiomyocyte apoptosis.
出处
《内蒙古医科大学学报》
2014年第6期527-531,共5页
Journal of Inner Mongolia Medical University
基金
内蒙古医科大学青年创新基金项目(YKD2012QNCX007)
关键词
匝迪阿瓦
应激
心肌损伤
炎性介质
细胞凋亡
Za Di Aava
Stress
Myocardial injury
Inflammatory mediators
Cell apoptosis
作者简介
包龙棠（1981-），女，内蒙古医科大学附属医院主治医师。
通讯作者：周丽华，主任医师，硕士研究生导师E—mail：xiuliua@163．com内蒙古医科大学附属医院，010050
