摘要
目的观察法舒地尔对小鼠脓毒症所致急性肺损伤的影响及其使用与否对肺组织中HMGB1表达量的影响。方法将48只BALB-C小鼠随机分为对照组、盲肠结扎穿刺(CLP)组、法舒地尔预处理组,在6、24 h时间点取血、肺泡灌洗液及鼠肺用于结果分析。结果在6、24 h时间点,法舒地尔可以显著降低CLP所致的小鼠血清中TNF-α及HMGB1和肺泡灌洗液中蛋白浓度的增加。除此之外,法舒地尔预处理还可以逆转CLP诱导的小鼠肺组织病理形态改变。同时,法舒地尔能减少CLP所致小鼠肺组织中HMGB1 mRNA表达量的增加。结论法舒地尔能减轻CLP小鼠所致的肺损伤并降低肺组织中HMGB1的表达。
Objective To observe the influence of fasudil on acute lung injury induced by sepsis in mice and the influence of using fasudil or not on the expression quality of HMGB1. Methods 48 BALB-C mice were randomly divided into the control group,the cecal ligation and puncture（CLP） group,the fasudil preconditioning group.Blood,bronchoalveolar lavage fluid and lung of mice was taken respectively at 6 and 24 hours time point for the analysis of results. Results At 6 and 24 hours time point,the increased TNF-α and HMGB1 level in serum and protein concentration in bronchoalveolar lavage fluid induced by CLP could be significantly reduced.In addition,pathological changes of lung tissue in mice was reversed by the fasudil preconditioning.At the same time,increased the expression quality of HMGB1 m RNA induced by CLP lung tissue in mice were reduced by fasudil. Conclusion Fasudil can alleviate the lung injury induced by CLP in mice and reduce the expression of HMGB1 of lung tissue.
出处
《中国当代医药》
2015年第6期4-7,共4页
China Modern Medicine
作者简介
赵振（1989-），男，2012级在读硕士研究生，急诊医学专业（重症方面）
通讯作者：韦广粤（1964-），男，主任医师，教授，硕士研究生导师．研究方向：呼吸机的临床应用
