摘要
[目的]探讨发酵床养猪模式下不同使用年限微生物群落的变化以及不同使用时间、不同深度的发酵床垫料中垫料组分的变化。[方法]采用常规分析方法测定样品中的营养成分含量,对不同年限样品中微生物分离提纯后,结合16S rRNA分子生物学鉴定对其微生物群落进行分析。[结果]随着发酵床使用时间的延长,垫料中的总氮、总磷、钾、钙、粗灰分和粗蛋白含量均显著增加,而水分含量降低不明显;使用1年和2年的垫料从上层到下层总氮、总磷、总钾和钙的浓度逐渐降低,不同使用时间、不同深度发酵床垫料中粪尿组水分、总氮、总磷、钾、钙、粗灰分、粗蛋白均高于其他组。随着使用年限的增加,猪生物发酵床垫料中微生物群落的多样性有降低的趋势。芽孢杆菌属细菌和梭菌属细菌在1年期样品和2年期样品中都是优势菌,对猪生物发酵床垫料中有机质的降解发挥着重要的作用。[结论]该研究可为垫料的资源化合理利用提供理论依据。
[ Objective] The research aimed to discuss the changes of microbial community with different years under the swine raising model of fermentation bed and the components in the beddings of fermentation bed with different depth in different years. [ Method ] The contents of nutri- tional components in the samples were determined by using conventional analysis method. The microbe were isolated and purified from samples with different years. Based on 16S rRNA molecular biological identification, the microbial community was analyzed. [ Result] With the prolon- ging the using years of fermentation bed, the contents of total nitrogen, total phosphorus, K, Ca, crude ash and crude protein in the beddings were significantly increased, but the water content had no obvious decrease. The concentrations of total nitrogen,total phosphors, total K and Ca in the bedding with one using years and two using years were gradually decreased from the upper layer to the ｜ower layer. The contents of water, total nitrogen,total phosphors, K, Ca, crude ash and crude protein in the beddings with different depth and different using years in the group of feces and urine were higher than that in other groups. With the increasing the using years, the diversity of microbial community in the beddings of swine micro-fermentation bed had the trend of decreasing. Bacillus bacteria and Clostridium bacteria were dominant bacteria in the first and second year of samples, which played an important role in depredating organic matter in micro-fermentation beddings. [ Conclusion ] The research could provide theoretical basis for the rational utilization of beddings resources.
出处
《安徽农业科学》
CAS
2015年第8期98-99,101,共3页
Journal of Anhui Agricultural Sciences
基金
嘉兴市科技计划项目(2011AY1043)
关键词
发酵床
微生物
多样性
成分
Bio-fermentation bed
Microbial community
Diversity
Nutritional components
作者简介
赵国华（1980-），女，山西运城人，讲师，博士，从事固体废物处理与资源化研究。
通讯作者，助理研究员，博士，从事农业生态方面的研究．
