摘要
目的:探索腹腔注射表没食子儿茶素没食子酸酯(EGCG)对小鼠运动疲劳的拮抗作用。方法:将120只小鼠随机分为生理盐水对照(1 ml/kg·d)(A组)、EGCG低浓度组(10 mg/kg·d)(B组)、EGCG高浓度防护组(50 mg/kg·d)(C组)(n=40)。建立疲劳小鼠训练模型,每天腹腔注射不同剂量的EGCG或生理盐水,持续给药28 d。检测小鼠负重游泳、轮转耐力、爬杆、动物耐缺氧时间及血乳酸水平(BLA)、血尿素氮(BUN)、血乳酸脱氢酶(LDH)及肝糖原(LG)、肌糖原(MG)等疲劳相关指标。结果:腹腔注射EGCG组(B组、C组)小鼠负重游泳、爬杆、轮转耐力持续时间及耐缺氧存活时间显著延长,且较对照组相比具有显著性差异(P<0.05,P<0.01);同时提高小鼠血清中LDH浓度,降低BLA、BUN浓度及升高MG和LG含量。高浓度EGCG的抗疲劳效果与低浓度组相比更为明显(P<0.05)。结论:EGCG具有拮抗小鼠运动疲劳的作用。
Objective： To investigate the effects of epigallocatechin gallate（ EGCG） against exercise-induced fatigue in mice.Methods： Total 120 mice were randomly divided into three groups and tested separately. For each test,there were 30 mice subdivided into high dose（ 50 mg / kg·d EGCG） and low dose（ 10 mg / kg·d EGCG） groups as well as saline control group（ 1 ml / kg·d） with10 in each. Burden swimming,running wheel endurance,stick climbing and hypoxia tolerance exercise were used to establish fatigue mice training model in three groups. And intraperitoneal injection with different doses of EGCG per day for consecutively 28 days and the mice in the control group were treated with normal saline. After the last each test,the blood lactic acid（ BLA）,blood urea nitrogen（ BUN）,blood lactate dehydrogenase（ LDH）,muscle glycogen（ MG） and liver glycogen（ LG） of each group of mice were determined. Results： EGCG treatment groups（ B and C） revealed a prolonged the mice survival time of burden swimming test,hypoxia tolerance,running wheel time and the ability of stick climbing（ P〈 0. 05 or P 〈0. 01）,and increased LDH activity and MG and LG contents,reduced contents of BLA and BUN. High dose group had an obviously increase effect than lower dose group（ P〈 0. 05）. Conclusion： EGCG has significant effects against exercise-induced fatigue in mice.
出处
《中国应用生理学杂志》
CAS
CSCD
2015年第1期85-88,共4页
Chinese Journal of Applied Physiology
基金
中央高校基本科研业务费资助项目(CQDXWL-2012-100)
作者简介
【通讯作者】Tel：13658362808；E-mail：hcy@cqu．edu．cn
