摘要
目的 观察成年大鼠脊髓损伤后是否存在神经元再生. 方法 36只Wistar成年雌性大鼠按随机数字表法分为正常对照组（6只）和脊髓损伤组（30只）.采用Allen打击模型（25 g·cm）在T10段造成急性脊髓损伤,于损伤后3d、1,2,3,4周进行取材.取材前72 h开始经腹腔注射BrdU （300 mg·kg^-1·d^-1）,每12 h注射1次.取距离损伤中心10 mm内脊髓行NF-200和BrdU免疫荧光双标,观察其表达阳性细胞数量. 结果 正常对照组可见少量BrdU阳性细胞,未发现BrdU和NF-200双标阳性细胞.脊髓损伤组伤后3d可见大量BrdU阳性细胞出现;1周时BrdU阳性细胞数量达到高峰,并可见少量BrdU和NF-200双标阳性细胞;2周时BrdU和NF-200双标阳性细胞最多见,主要集中在距离损伤区约3～5 mm处;3周时BrdU和NF-200双标阳性细胞罕见;4周时均未发现BrdU和NF-200双标阳性细胞. 结论 大鼠脊髓损伤后在损伤部位附近存在神经元再生,为干细胞修复脊髓损伤提供新的思路.
Objective To observe the neuronal regeneration after spinal cord injury in adult rats.Methods Thirty-six adult Wistar rats were randomly divided into normal control group （n =6） and spinal cord injury group （n =30）.Allen＇s weight-drop method （25 g/cm） was adopted to produce acute spinal cord injury at T10 segment.Rats were sacrificed at 3 days and 1,2,3,and 4 weeks postinjury.BrdU was administered intraperitoneally to the rats 72 hours before operation,once per 12 hours.Double immunofluorescence staining of NF-200 and BrdU was performed at spinal sites within 10 mm away from the injury to detect the positive cells in both groups.Results A few BrdU-positive cells were founded in the normal control group,with no BrdU/NF-200 positive cells.In spinal cord injury group,BrdU-positive cells were abundant at day 3 and peaked at week 1 along with few Brdu/NF-200 positive cells ; Brdu/NF-200 positive cells showed a peak increase at week 2 with majority accumulated at area 3-5 mm away from the injury,were rare at week 3 and none at week 4.Conclusion Neuronal regeneration is detected in the vicinity of the injury,which provides new ideas for induction of endogenous stem cells to treat spinal cord injury.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2015年第2期164-168,共5页
Chinese Journal of Trauma
基金
国家自然科学基金资助项目（81272172）
国家临床重点专科建设资助项目（2013544）
关键词
脊髓损伤
神经元
干细胞
大鼠
Spinal cord injuries
Neurons
Stem cells
Rats
作者简介
通信作者：南国新，电话：13608397992，Email：ngx1215@126．com
