摘要
目的评价16种与阿尔茨海默病相关的天然药物提取物对β淀粉样蛋白(Aβ42)寡聚体致PC-12细胞毒性的作用。方法通过超声波破碎法获得16种天然药物的提取物,并筛选具有抑制Aβ42寡聚体诱导的神经毒性作用的药物。将PC-12细胞随机分为实验组、细胞损伤对照组、天然药物对照组、正常细胞对照组。通过细胞培养液中加入Aβ42寡聚体诱导建立PC-12细胞损伤模型。采用MTT比色法测定各组细胞存活率。结果苦参、石菖蒲、何首乌、远志、当归、女贞子、黄芪、益智仁、绞股蓝和淫羊藿10种天然药物提取物具有抑制Aβ42寡聚体诱导神经毒性的作用,其中苦参碱的提取物抑制作用最强。细胞损伤对照组PC-12细胞的存活率显著高于实验组、天然药物对照组及正常细胞对照组(P均<0.05)。结论最终确定10种天然药物(苦参、石菖蒲、何首乌、远志、当归、女贞子、黄芪、益智仁、绞股蓝和淫羊藿)提取物具有抑制Aβ42寡聚体诱导的神经毒性作用,苦参碱的提取物抑制作用最强。
Objective To evaluate the effects of 16 extracts of Alzheimer disease-related natural Chinese medicine on Aβ42 oligomer induced PC-12 cell toxicity.Methods The effective components of 16 natural Chinese medicines were extracted by ultrasonic method and the extracts which had the effect of inhibiting the cytotoxicity of Aβ42 were sreened.The PC-12 cells were randomly divided into the experimental group,cell damage group,natural medicine control group and normal cells control group.The extracts were added to the culture medium of the PC12 cell models respectively which was induced by the Aβ42 oligomers,MTT colorimetric analysis was used to determine the cell survival rate.Results Matrine,Shichangpu,Radix Polygoni Multiflori,Radix Polygalae,Chinese angelica,Ligustrum lucidum,astragalus root,Fructus alpiniae oxyphyllae,Gynostemma pentaphyllum and Epimedium,a total of 10 extracts had the effect of inhibiting the cytotoxicity of Aβ42.The extract of Matrine had the strongest effect.The survival rate in PC-12 cells of the cell damage group was significantly higher than that of the experimental group,natural medicine control group and normal cells control group（ all P〈 0.05）.Conclusion We finally identify 10 extracts（ Matrine,Shichangpu,Radix Polygoni Multiflori,Radix Polygalae,Chinese angelica,Ligustrum lucidum,astragalus root,Fructus alpiniae oxyphyllae,Gynostemma pentaphyllum and Epimedium） have the effect of inhibiting the cytotoxicity of Aβ42,and Matrine extract has the strongest effect.
出处
《山东医药》
CAS
2014年第9期4-6,30,共4页
Shandong Medical Journal
基金
广东省科技厅博士启动项目(S2013040013740)
广东医学院心脑相关广东省重点实验室开放基金项目(GDKLARCCD201301)
广东医学院科研基金博士启动项目(S2013040013740)
广东中医药局2013建设中医药强省科研课题项目(20131257)
广东省医学科研基金项目(B2013306)
作者简介
崔理立（1983-），男，助理研究员，主要研究方向为神经变性疾病发生的分子机制。E—mail：Lilicui1983@aliyun．com
通信作者：张应玖（1962-），女，教授，博士生导师，主要研究方向为神经细胞损伤与修复。E-mail：yingjiu@jlu．edu．cn
