摘要
以薰衣草花为原料,经体积分数为70%乙醇提取,将提取物浓缩成浸膏后用水分散,再依次用石油醚、氯仿、乙酸乙酯和正丁醇依次萃取。以L-DOPA为底物测定石油醚、氯仿、乙酸乙酯和正丁醇萃取部分及萃取后水相部分对酪氨酸酶的抑制作用。实验结果表明,薰衣草花水相萃取物对酪氨酸酶有较强的抑制作用,IC50为0.96 g/L,动力学研究表明,薰衣草花水相萃取物对酪氨酸酶的抑制作用是可逆的,其抑制类型为线性混合型抑制,对游离酶和酶-底物络合物的抑制常数分别为3.00和12.00 g/L。
Dried flower of lavender was used as raw material and it was extracted with 70%（ volume fraction）ethanol. The extract was concentrated to concrete and dispersed in water. Then the dispersion was further extracted with petroleum ether,chloroform,ethyl acetate and n- butanol successively. The extracts from the petroleum ether,chloroform,ethyl acetate and n- butanol as well as the water phase raffinate were assayed for tyrosinase inhibitory effects using L- DOPA as substrate. Results showed that the water extract demonstrates significant inhibitory effects on tyrosinase activity. The IC50 value is 0. 96 g / L. Kinetic study showed that the inhibitory mechanism of water- soluble extract is reversible,and the inhibitory type is the linear mixed- type inhibition. The inhibition constants of the free enzyme and the enzyme- substrate complex were 3. 00 and 12. 00 g / L,respectively.
出处
《日用化学工业》
CAS
CSCD
北大核心
2015年第2期90-93,共4页
China Surfactant Detergent & Cosmetics
基金
国家自然科学基金资助项目(21365018)
新疆生产建设兵团科技攻关资助项目(2011AB030)
作者简介
罗碧（1988-），女，陕西人，硕士研究生，E—mail：lb．cold@aliyun.com。
通讯联系人：刘文杰，副教授，电话：（0997）4681610，E—mail：lug@sina．com
