摘要
目的:探讨硫化氢(H2S)对原代肝纤维化鼠肝细胞增殖、凋亡的作用及其与磷酯酰肌醇3激酶(phosphoinositide-3-kinase,PI3K)/Akt通路的关系.方法:采用CCl4复合因素法制备肝纤维化大鼠模型,胰酶两步灌流法提取肝纤维化鼠肝细胞,设对照组、Na HS组、Na HS+LY294002组,M T T法测定N a H S和LY294002对大鼠肝细胞的增殖抑制率(inhibition rate),H o c h e s t33342法检测各组肝细胞的凋亡率,Western blot检测PI3K、Phospho-Bad蛋白的表达量.结果:50μmol/L NaH S、50μmol/L LY294002为最适浓度;与对照组相比,Na HS+LY294002组细胞凋亡率高(P<0.05),Na HS组细胞凋亡率低,但差异无统计学意义;NaH S组PI3K、PhosphoBad蛋白表达量高于对照组和NaH S+LY294002组,而Na HS+LY294002组PI3K、Phospho-Bad蛋白表达量低于对照组和Na HS组,各组间相互比较差异均有统计学意义(P<0.05).结论:H2S可以通过激活PI3K/Akt通路促进肝细胞的增殖,抑制肝细胞的凋亡.
AIM： To explore the effect of exogenous hydrogen sulfide on rat hepatocyte proliferation and apoptosis and the role of phosphoinositide-3-kinase （PI3K）/Akt pathway.
METHODS： Female SD rats were subcutaneously injected with carbon tetrachloride to induce hepatic fibrosis, and hepatocytes were isolated from the rats. Primary hepatocytes were divided into three groups： a normal control group, a hydrogen sulfide group, and an LY294002 plus hydrogen sulfide group. MTT method was used to determine cell proliferation. Apoptosis of liver cells was detected by Hoechst 33342 staining. The expression of PI3K and phosphorylated Bad protein was detected by Western blot.
RESULTS： Sodium hydrosulfide at 50 μmol/L promoted cell proliferation （P 〈 0.05, compared with the control group）, while 50 μmol/L LY294002 inhibited cell proliferation （P 〈 0.05, compared with the control group）. Compared with the control group and sodium hydrosulfide group, the LY294002 plus hydrogen sulfide group had a significantly higher rate of cell apoptosis （P 〈 0.05）. The expression of PI3K and phosphorylated Bad protein in the sodium hydrosulfide group was significantly higher than that in the control group and LY294002 plus hydrogen sulfide group （P 〈 0.05）. However, PI3K and phospho-Bad protein expression in the LY294002 plus hydrogen sulfide group was significantly less than in the control group （P 〈 0.05）.
CONCLUSION： Hydrogen sulfide promotes primary hepatocyte proliferation and inhibits its apoptosis possibly through the PI3K/Akt pathway.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第36期5602-5608,共7页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.81170402~~
作者简介
岳雅伦，在读硕士，主要从事慢性肝病及消化系肿瘤的研究．
郑勇，教授，主任医师，832008，新疆维吾尔自治区石河子市北二路，石河子大学医学院第一附属医院消化内科．zy2850@126．com
