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利用异源酵母功能互补法研究水稻铵转运体OsAMT1;1功能及调控机制 被引量:1

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摘要 水稻是一种以铵态氮为主要氮素营养的重要粮食作物,存在至少12个铵转运体,对水稻铵的吸收起着至关重要的作用,其中,水稻铵转运体1;1(Oryza sativa ammonium transporter1;1,Os AMT1;1)是一个在根部和地上部相对组成型表达的基因。通过异源酵母功能互补法研究水稻铵转运体Os AMT1;1的功能及其调控机制,结果表明,Os AMT1;1是一个功能型的铵转运体,和铵的同系物甲基铵(Methylammonium,Me A+)相比,Os AMT1;1对铵具有相对较高的选择性;Os AMT1;1介导铵的吸收不依赖于外界质子,转运的底物可能是铵离子;Os AMT1;1介导吸收铵的过程是一个依赖能量的主动运输过程,对铵的吸收可能不受钙离子参与的磷酸化过程调控。
出处 《江苏农业科学》 北大核心 2015年第1期27-31,共5页 Jiangsu Agricultural Sciences
基金 国家自然科学基金重大研究计划(编号:91125028)
作者简介 杨顺瑛(1982-),女,湖北恩施人,博士,主要从事分子植物营养学研究。Tel:(025)86881553;E—mail:ysy@issas.ac.cn。 通信作者:苏彦华,山东济宁人,博士,教授、研究员,从事植物营养与分子生物学研究。E—mail:yhsu@issas.ac.cn。
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