摘要
目的：研究甘草渣中的总黄酮体外对羟自由基（·OH）的清除作用。方法将甘草渣中的总黄酮提取物制成不同浓度溶液，利用电子顺磁共振（EPR）技术检测甘草渣中总黄酮对体外产生羟基自由基（·OH）的清除率。结果甘草渣中的总黄酮在0．25～25 mg／mL 范围内对·OH 自由基清除率随浓度增加呈上升趋势，分别为50．53％、59．89％、65．64％、69．30％、80．06％。结论甘草渣中的总黄酮有明显的清除羟自由基的作用，EPR技术和试验结果可用于甘草渣中黄酮类物质有效活性成分的初步筛选。
Objective To study the scavenging capacity on hydroxyl radical of the total flavonoids in licorice waste residue.Methods The total flavone extract from licorice residue was made into solutions of different concentrations and the removal rate of total flavonoids of licorice residue was determined by electron para-magnetic resonance （EPR）detection technology.Results When the residue of Glycyrrhiza total flavones was in the range of 0.25-25 mg/mL.the hydroxyl radical scavenging rate is on the rise with the increase of concentration,respectively 50.53%,59.89%,65.64%,69.30% and 80.06%.Conclusion Total flavonoids from licorice slag has obvious scavenging effect on hydroxyl free radical.EPR technology and the test results can be used in preliminary screening of active compositions of flavonoids in licorice slag material.
出处
《新疆医科大学学报》
CAS
2015年第2期182-183,186,共3页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区科技支撑计划项目(201333102)
关键词
甘草渣
黄酮
电子顺磁共振
羟基自由基
licorice waste residue
flavonoids
electron paramagnetic resonance （EPR）
hydroxyl radical
作者简介
李鸿飞（1979-），男，本科，主管中药师，研究方向：医院药学研究。
通信作者：倪慧，女，研究员，硕士研究生导师，研究方向：天然药物的分析及制剂，E—mail：qingdegang@sina．com。
