摘要
应用RT-PCR方法对猪瘟病毒E0基因进行扩增、克隆及测序,用DNA Star分析软件对6株毒株与猪瘟兔化弱毒苗（HCLV）、石门（Shimen）株、Paderborn株、GXWZ02株的相应片段进行比较分析,构建了CSFV遗传进化树。序列分析表明,广西流行株与HCLV株、Shimen株、Paderborn株、GXWZ02株之间核苷酸的同源性分别为80.8%-81.5%、82.8%-83.3%、93.1%-94.2%、93.7%-94.2%,推导的氨基酸同源性分别为88.3%-89.7%、89.7%-91.1%、96.2%-97.7%、97.7%-99.1%;6株广西CSFV流行毒株与国内外已发表的14株病毒相应序列进行比较,构建遗传进化树,结果表明所比较的20株毒株分为2个基因群,广西流行毒株均属于基因群Ⅱ。本研究成功地对广西流行猪瘟病毒株的E0基因进行了测序分析,表明近年来广西流行毒株未发生较大的变异,但病毒株有远离疫苗株发展的趋势。
Using RT-PCR method,E0 gene of six CSFV strains were amplified,cloned and sequenced. Then the sequences were analyzed and compared with the same gene sequences of HCLV strain,Shimen strain,Paderborn strain and GXWZ02 strain by DNAstar software. The nucleotide homologies were 80.8%-81.5%,82.8%-83.3%,93.1%-94.2% and 93.7%-94.2%, respectively, and amino acid w ere 88.3%-89.7%,89.7%-91.1%,96.2%-97.7% and 97.7%-99.1% with reference stains.The phylogenet-ic analysis showed that GX strains and other 14 strains were classified as 2 groups,while all the GX strains belonged to genotype Ⅱ.The study successfully sequenced the E0 gene of six CSFV strains in Guangxi. The results revealed that there is no large variation in Guangxi pandemic strains in recent years,but a trend that virus development is far away from vaccine strain.
出处
《动物医学进展》
北大核心
2015年第1期31-35,共5页
Progress In Veterinary Medicine
基金
南宁市科技攻关项目(南桂20142078)
关键词
猪瘟病毒
E0基因
序列分析
同源性比较
Classical swine fever virus
E0 gene
sequence analysis
homology
作者简介
曾咏芳（1988-），女，广西浦北人，硕士研究生，主要从事动物分子病原学研究。
通讯作者
