摘要
目的采用气相色谱-质谱联用(GC-MS)技术对大肠风与小肠风挥发油进行对比分析,为二者合理利用提供参考依据。方法水蒸气蒸馏提取挥发油。色谱柱为HP-5MS石英毛细管色谱柱(30 m×0.25 mm,0.25μm);程序升温;载气为氦气(He),体积流量为1.0 m L/min;检测器温度为280℃;溶剂延迟为3 min;进样量为0.2μL;分流比为100∶1;空气流速为300 m L/min;尾吹气N2流速为30 m L/min。结果用水蒸气蒸馏法提取所得挥发油成分较复杂,大肠风与小肠风挥发油共有成分为石竹烯、α-石竹烯、斯巴醇、反式-橙花叔醇、α-荜澄茄油烯等14个,主要为烯烃类及醇类;大肠风特有成分为(-)-β-榄香烯、(+)-β-蛇床烯、1,5,5-三甲基-6-亚甲基-环己烯,含量都高达14%以上;小肠风中(-)-斯巴醇、反式-橙花叔醇,含量都高达15%以上。结论大肠风与小肠风挥发性成分具有差异性,可作为鉴别二者的基本依据。
Objective To analyze volatile oil from the plants of Piper boehmeriifolium（Miq.） Wall. ex C. DC. and Piper hancei Maxim. Methods Volatile oils were exacted by the steam distillation（SD） method. HP-5 capillary column（30 m×0.25 mm, 0.25 μm）; temperature programming; Nitrogen as the carrier gas; Volume flow was 1.0 m L/min; detecror tempertature was 280℃; solvent delay time was 3 min; injection volume was 0.2 μL; split ratio was 100∶1; the velocity of air was 300 m L/min; the velocity of N2 was 30 m L/min. Results The yield of volatile oil was high. Their ingredients were complicated, which were including 14 main cornmon chemical compositions, such as Caryophyllene, α-Caryophyllene, Spathulenol,（E）-Nerolidol, α-Cubebene and so on. The specific components of Piper boehmeriifolium（Miq.） Wall. ex C. DC. were（-）-β-Elemen,（＋）-β-Selinene, 1, 5, 5-Trimethyl-6-methylene-cyclohexene. The contents were more then 14%; the specific components of Piper hancei Maxim. were（-）-Spathulenol and（E）-Nerolidol,the contents were more than 15%. Conclusion Main components are differences between Piper boehmeriifolium（Miq.）Wall. ex C. DC. and Piper hancei Maxim., it might be the basis for variety identification.
出处
《中国医药导报》
CAS
2014年第36期83-87,共5页
China Medical Herald
基金
广西壮族自治区自然科学基金项目(编号2011GXNSFF018006)
广西壮族自治区瑶族习用药材质量评价与标准研究项目(编号MZY2012039)
广西壮族自治区首批"2011协同创新中心"项目
关键词
大肠风
小肠风
挥发油
气相色谱-质谱联用
Piper boehmeriifolium（Miq.） Wall.ex C.DC.
Piper hancei Maxim.
Volatile oil
GC-MS
作者简介
【作者简介】苏玲（1983-），女，硕士，主要从事中药鉴定及质量控制研究。
通讯作者
