摘要
化性是家蚕的重要生理特性,受第6染色体上的化性主基因V及Z染色体上的伴性成熟基因Lm的影响,伴性早熟基因Lme促进家蚕化性趋向多化性。采用多化性家蚕品种nistari和二化性家蚕品种p50配制BC1M群体[p50×(nistari×p50)],选择其中734头产非滞育卵的雌蛾个体作为定位群体,以其基因组DNA为模板进行SSR标记的PCR扩增,应用高分辨率熔解曲线分析(HRM)技术进行各个标记位点的基因型分析,绘制出遗传总距离为12.91 c M的分子遗传连锁图,Lme基因位于标记s2734-299和s2734-525之间,与2个标记的遗传距离均为0.41 c M。s2734-299和s2734-525间的物理距离为226 kb,其间有18个预测基因,有2个属于DNA序列特异性结合转录因子基因,但尚不能判定这2个基因是否与Lme有明确的关联。
Voltinism is an important physiological characteristic of silkworm（ Bombyx mori）,which is affected by the dominant V gene located on chromosome 6 and the sex-linked maturation gene（ Lm） located on sex chromosome Z. The sexlinked early maturation gene（ Lm^e） could promote change of silkworm voltinism towards multivoltinism. Here,silkworm multivoltine variety nistari and bivoltine variety p50 were used as parental strains to construct BC1 M population [p50 ×（ nistari × p50） ],among which 734 female moths laying non-diapause eggs were selected as mapping group for PCR amplification of their genomic DNAs. SSR molecular markers were genotyped by high-resolution melting（ HRM） analysis technique. A map covered a total genetic distance of 12. 91 c M was obtained. Lmewas located between markers S2734-299 and S734-525,and the genetic distances between Lmeand these two markers was both 0. 41 c M. Physical distance between the two markers was 226 kb. There were altogether 18 predicted genes in this region. However,two genes coding for DNA-binding specific transcription factors cannot be determined as associated genes to Lm^e.
出处
《蚕业科学》
CAS
CSCD
北大核心
2014年第6期995-999,共5页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金项目(No.31272499)
作者简介
余俊杰（1988-），女，硕士研究生。E-mail：dalianma0721901@sina．com
通信作者信息：张国政，研究员。E—mail：zgzsri@163．com.Corresponding author．E—mail：zgzsfi@163．com
