摘要
目的建立酶学参考方法不确定度研究模型,系统地对丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、α-淀粉酶(AMY)、谷氨酰基转移酶(GGT)和肌酸激酶(CK)6个酶学参考方法的不确定度影响因素进行研究,以明确各参考方法影响的关键因素,更好地指导参考方法的建立和运行。方法参照JJF1059-1999《测量不确定度评定与表示》、JJF1135-2005《化学分析测量不确定度评定》、CNAS-GL06《化学分析中不确定度的评估指南》和CNAS-CL33《检测和校准实验室能力认可准则在临床酶学参考测量领域的应用说明》,采用理论评估和实验设计相结合的方法对6个酶学参考方法不确定度进行评定。结果建立的酶学不确定度评定模型为C=ΔAΔt·1ε·1L·V1+V2+VSVS·fT·fpH·fλ·f试剂·f测试结果。结论建立了酶学参考方法不确定度的评定模型,并成功运用于6个酶学参考方法不确定度的评定。
Objective To develop an uncertainty study model of the enzymological reference method to systematically research the uncertainty influencing factors of 6 enzymological reference methods ALT, AST, LDH, AMY, GGT and CK for determining the key factors influencing the reference method and better guiding the establishment and operation of the enzymological reference methods. Methods The uncertainty of the six enzymological reference methods was evaluated by the theoretical evaluation combined with the experiment design according to series of standards including JJF1059-1999 Evaluation and Expression of Uncertainty in Measurement,JJFl135-2005 Evaluation of Uncertainty in Chemical Analytical Measurement, CANA-GL06 Guidance on Evaluating the Uncertainty in Chemical Analysis and CNAS-CL33 Guidance on the Application of Testing and Calibration Laboratories Competence Accreditation Criteria in the Field of Clinical Enzymology Reference Measurement. Results The established enzymological uncertainty study model was C=△A/△t· 1/ε·1/L· V1 ＋V2 ＋Vs/Vs·fT·fpH·fλ · fkit· fresult·Conclusion The uncertainty evaluation model is set up and successfully applied in the uncertainty evaluation of the six enzymological reference methods
出处
《国际检验医学杂志》
CAS
2015年第1期77-79,共3页
International Journal of Laboratory Medicine
关键词
酶学参考方法
不确定度
不确定度评定
enzymological reference method
uncertainty
uncertainty evaluation
作者简介
陈明，男，检验技师，主要从事临床检验标准化研究。
