摘要
铃兰(Convallaria majalis L.),属于百合科铃兰属多年生宿根花卉,铃兰的种子采集困难,且分株繁殖系数低,利用再生离体培养技术可在短期内获得大量品质优良的植株,满足国内外市场需求。本研究用不同外植体在MS培养基附加低浓度的激素诱导愈伤组织,经过分化和增殖培养获得无菌根苗,在含有适宜浓度激素水平生根培养基上使其生根,提高生根率。通过优化组合和重复试验,筛选快繁体系,对铃兰种质资源的保护和开发利用具有一定的参考意义。
Convallaria majalis L.is a perennial plant,which belongs to the convallaria L.in liliaceae family.Because the seed of Convallaria majalis L.collection is difficulty,and division propagation coefficient is lower,but using of regeneration in vitro can obtain a large number of high quality plants in the short term,and meet the market demand at home and abroad.Different explants in MS medium with lower hormone concentration is used to induce callus in this experiment,obtain aseptic seedlings after differentiation and proliferation,and improve the rootage rate in the medium containing the appropriate concentration.Rapid propagation system is screened through optimizing combination and repeated experiment,which is of certain practical significance to the protection and utilization of germplasm resources of Convallaria majalis L.
出处
《西昌学院学报（自然科学版）》
2014年第4期14-16,共3页
Journal of Xichang University(Natural Science Edition)
基金
安徽省优秀青年人才基金项目(项目编号:2011SQRL194ZD)
关键词
铃兰
外植体
愈伤组织
分化
Convallaria majalis L.
explants
callus
differentiation
作者简介
李东林（1979-），男，硕士研究生，副教授，主要从事植物生产与生理生态学研究。
