摘要
目的探讨年龄因素对高龄不孕患者接受辅助生殖技术(ART)后胚胎发育情况及妊娠结局的影响。方法选择2013年7月至2014年1月于内蒙古医科大学附属医院生殖中心行体外受精-胚胎移植(IVF-ET)及卵胞浆内单精子显微注射(ICSI)的不孕患者155例为研究对象,共计184个周期。按照年龄,将纳入患者分为3组:Ⅰ组(n=67,周期数为63,35岁≤年龄<37岁),Ⅱ组(n=60,周期数为55,37岁≤年龄<40岁),Ⅲ组(n=57,周期数为37,年龄≥40岁)。各组患者身高、体质指数、不孕年限及受孕史等比较,差异无统计学意义(P>0.05)。本研究遵循的程序符合内蒙古医科大学附属医院人体试验委员会制定的伦理学标准,得到该委员会批准,分组征得受试对象知情同意,并与之签署临床研究知情同意书。结果 1Ⅲ组接受IVF-ET和ICSI者的新鲜胚胎妊娠率较Ⅰ组显著降低,且差异有统计学意义(P<0.05),但与Ⅱ组比较,差异无统计学意义(P>0.05)。3组间接受IVF-ET和ICSI者的冷冻胚胎妊娠率及胚胎冷冻率比较,差异均无统计学意义(P>0.05)。2Ⅲ组妊娠率与胚胎种植率均较Ⅰ组及Ⅱ组显著降低,且差异均有统计学意义(P<0.05);Ⅱ组妊娠率较Ⅰ组显著降低,且差异有统计学意义(P<0.05),但其胚胎种植率与Ⅰ组比较,差异无统计学意义(P>0.05);3组间受精率、卵裂率、优质胚胎率、优质胚胎移植率、自然流产率及活婴出生率比较,差异均无统计学意义(P>0.05)。3相同ART下,3组间受精率、卵裂率及优质胚胎率比较,差异均无统计学意义(P>0.05)。结论年龄因素可影响高龄不孕患者胚胎着床及妊娠成功。
Objective To analyze factor of age effect on embryonic development and pregnancy outcomes in elderly infertile women undergoing assisted reproductive technology（ART）. Methods A total of 155 infertile patients who were undergoing in vitro fertilization and embryo transfer（IVF-ET） and intracytoplasmic sperm injection （ICSI） in Reproductive Medical Center, Affiliated Hospital of Inner Mongolia Medical University from July 2013 to January 2014 were chosen as study subjects, and total cycles were 184. According to age,they were divided into three groups：group Ⅰ （ n=67,63 cycles,35 years old≤ age〈37 years old）,group Ⅱ （n= 60,55 cycles, 37 years old ≤age〈40 years old） and group Ⅲ （n= 57, 37 cycles,age≥40 years old）. There were no statistically significant differences among three groups in general clinical data such as body height, body mass index ,years of infertility and pregnancy history （P〉 0.05）. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Affiliated Hospital of Inner Mongolia Medical University. Informed consent was obtained from each participant. Results （1）Fresh embryos pregnancy rates of IVF-ET and ICSI in group Ⅲ were significantly lower than those in group Ⅰ ,and there were significant differences（P〈0. 05）. However, there were no significant differences between group Ⅲ and group Ⅱ in fresh embryos pregnancy rates by IVF-ET and ICSI （P〉0.05）. There were no significant differences among three groups in frozen embryo pregnancy rates and embryo freezing rates by IVF-ET and ICSI （P〉0.05）. （2）Pregnancy rate and embryo implantation rate in group Ⅲ were significantly lower than those in group Ⅰ and Ⅱ ,there were significant differences （P〈0.05） ; Pregnancy rate in group Ⅱ was significantly lower than that in group Ⅰ ,and there was significant difference（P〈0.05） ,but there were no significant differences between group Ⅱ and group Ⅰ in embryo implantation rate（P〉0.05） ; There were no significant differences among three groups in fertilization rate, cleavage rate, high quality embryo rate, high quality embryo transplant rate, spontaneous abortion rate and live birth rate（P〉0.05）. （3）There were no significant differences among three groups in fertilization rate, cleavage rate and high quality embryo rate undergoing same ART（P〉0.05）. Conclusion Factor of age can effect the successful rates of embryo implantation and pregnancy of elderly infertile women.
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2014年第6期64-67,共4页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
内蒙古自治区自然科学基金项目(2013MS1137)~~
关键词
生殖技术
辅助
体外受精
精子注射
细胞浆内
胚胎植入
女性
Reproductive techniques, assisted
Fertilization in vitro
Sperm injections,intracytoplasmic
Embryo implantation
Women
作者简介
通信作者：梁琳，Email：ndsky30311007@163．com
