摘要
通过引物筛选获得18条RAPD多态性引物,利用该引物对72份咖啡种质资源进行RAPD扩增,共扩增出149条条带,其中多态性条带126条,多态性位点为84.6%。运用UPGMA方法构建了聚类图,在相似系数0.632的水平下可将72份资源聚为3个大类,其中A类群包含了所有的中粒种资源(Coffea canephora Pierre)及一份由云南德宏所选育的中小粒种杂交种(阿拉伯斯塔),共34份咖啡种质资源;B类群包括了6份查理种(Coffea excelsa Chevalier)和大粒种(Coffea liberica Bull ex Hiern);C类群由小粒种(Coffea arabica.Linne)咖啡组成。结果说明咖啡种质的遗传关系种间容易划分,在种的分类水平上存在遗传关系多样性,部分资源的分类学地位与地理来源无相关性。
The genetic diversity of 72 coffee germplasm resources was analyzed by RAPD amplification using 18 primers,and a total of 149 bands were amplified,including 126 polymorphic bands with polymorphic loci 84.6％.Based on a clustering graph using UPGMA,72 resources could be divided into 3 categories at the similarity level of 0.632 coeficient.A total of 34 resources in C.canephora and a hybrid from Dehong,Yunnan were in Group A.Six resources in C.excelsa and C.liberica were in Group B.The left in C.arabica were in Group C.The results showed that the coffee genetic relationship of species could be easily divided,a genetic diversity existed and taxonomic status of resources had no correlation with geographical origin.
出处
《热带作物学报》
CSCD
北大核心
2014年第12期2313-2319,共7页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.31000740)
热带作物种质资源保护项目(No.14RZZY-49)
作者简介
黄丽芳（1985年-）,女,硕士;研究方向：热带作物遗传育种.
闫林（YANLin）,E-mail：yanlin2575@163.com.
