摘要
为探明养殖欧洲鳗鲡不同体位及其养殖水体中可培养菌群的组成结构,本研究利用16S rDNA序列分析法对从精养池鳗鲡的鳃部、肠道和表皮及其养殖水体中分离得到的可培养细菌进行了分子鉴定并构建了系统发育树。研究结果显示,鳃部、肠道、表皮和水体的菌密度分别为1.6×106 cfu/g、2.2×107 cfu/g、1.4×104cfu/cm2和4.5×103 cfu/mL;分离菌株分别属于γ-变形菌纲的肠杆菌属、不动杆菌属、栖水菌属,β-变形菌纲的食酸菌属,芽孢杆菌纲的葡萄球菌属、气球菌属,黄杆菌纲的金黄杆菌属和放线菌纲的微球菌属等5大类8个菌属。其中,微球菌属、肠杆菌属和栖水菌属分布最广,各样品中均有检出;而气球菌属和食酸菌属仅在鳃部分布。各生态位中,鳃部菌群最为多样,含有葡萄球菌属之外的7个属;而水体菌群种类最少,只有4个属。此外,菌群组成含量的分析结果表明,鳗鲡鳃部以金黄杆菌属(28.3%)和肠杆菌属(26.1%)居多;而肠道、表皮和养殖水体都以微球菌属占绝对优势,分别为43.6%、53.5%和74.8%。
The 16S rDNA genes amplified from bacteria isolated from the gill, intestinal tract and skin of European eel（Anguilla anguilla） and the surrounding water were subjected to sequencing and phylogenetic analysis to ascertain the taxonomic composition of the culturable bacteria. The total bacterial load in the gill, gut, skin and the water was 1.6×10^6cfu/g, 2.2×10^7cfu/g, 1.4×10^4cfu/cm2and 4.5×10^3cfu/mL, respectively. The isolates belonged to Enterobacter, Acinetobacter, Enhydrobacter in γ-Proteobacteria group, Acidovorax in β-Proteobacteria group, Staphylococcus, Aerococcus in Bacilli group, Chryseobacterium in Flavobacteria group and Micrococcus in Actinobacteria group. Among these genera, Micrococcus, Enterobacter and Enhydrobacter were found in all the samples, while Aerococcus and Acidovorax just appeared in the gill. The organisms in the gill were the most diverse, whereas the least in the pond water. Chryseobacterium（28.3%） and Enterobacter（26.1%） were the most abundant genera in the gill while Micrococcus was the predominant in the gut（43.6%）, skin（53.5%） and the water（74.8%）.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2014年第2期307-313,共7页
Genomics and Applied Biology
基金
公益性行业(农业)科研专项(201203085)
国家自然科学基金(31202030)
中国科学院城市环境与健康重点实验室开放课题(KLUEH201106)
国家级大学生创新创业训练计划项目(Z81236)共同资助
关键词
鳗鲡
水体
可培养细菌
菌群组成
16S
RDNA
Anguilla anguilla
Pond water
Culturable bacteria
Taxonomic composition
16S rDNA
作者简介
共同第一作者
通讯作者，linmao@jmu．edu．cn
