摘要
利用阳离子淀粉制备了阳离子淀粉/质粒DNA纳米传递系统,通过凝胶电泳、激光粒度分析和原子力显微技术研究了不同取代度和N/P(氮/磷)摩尔比对传递系统复合性能和形貌特征的影响;进一步研究了DNase I内切酶消化液、模拟体液和溶菌酶溶液等不同模拟体内环境对阳离子淀粉/质粒DNA传递系统稳定性的影响规律,并考察了阳离子淀粉与质粒DNA复合性能随细胞内涵体pH变化的规律。结果显示,较高N/P比和阳离子取代度下的阳离子淀粉/质粒DNA传递系统复合性更好,抗DNase I和抗溶菌酶能力更强,且质粒DNA传递系统能够抵御pH=1.2的酸性环境,而在pH=4.0和pH=7.4的环境下N/P摩尔比>5时,能保护质粒DNA使其不被酸性环境破坏。
Cationic starch/plasmid DNA delivery system was prepared and the influence of degree of substitution and N/P mole ratio on binding capacity, particle morphology of the cationic starch/gene system was investigated by gel electrophoresis, particle size analysis and atomic force microscope. Furthermore, the protection of plasmid DNA and the stability of cationic starch/plasmid DNA complex delivery system in DNase I solution, simulated body fluid and lysozyme solution were evaluated and the change of binding capacity between cationic starch and pAcGFP1-C1 was also determined with pH in endosome. The results indicate that as the degree of cationic groups and N/P mole ratio increase, not only cationic starch and plasmid DNA interacted compact results in good stability of the cationic starch/DNA complex delivery system, but also the DNase I and lysozyme resistance of cationic starch/plasmid DNA complex delivery systems becomes stronger. The cationic starch/plasmid DNA complex nano-delivery systems are more stable in pH = 1.2, and when n （（N）/n （P）〉5, the plasmid DNA can be protected under the acid condition of pH = 4 and pH = 7.4 body fluid.
出处
《高分子材料科学与工程》
EI
CAS
CSCD
北大核心
2014年第7期137-142,共6页
Polymer Materials Science & Engineering
基金
国家自然科学基金资助项目(31271824
31130042
31071503)
华南理工大学中央高校基本科研业务费专项资金(2013ZG0009
2009ZZ0021)
教育部新世纪优秀人才支持计划项目(NCET-12-0193)
作者简介
通讯联系人：陈玲，主要从事碳水化合物化学与材料研究，E-mail：xxlee@scut．edu．cn
