摘要
以高抗CMV的烤烟品种台烟8号为母本(P1),以高感CMV的烤烟品种NC82为父本(P2),在2个不同的时间环境下构建P1、P2、F1和F24个世代群体,在植株不同生长时期进行CMV病害鉴定。运用植物数量性状"主基因+多基因"混合遗传模型分析方法对该世代群体的CMV抗性进行联合分析。结果表明,在温室环境中,苗期和成株期鉴定CMV抗性遗传都符合E1模型,即由2对加性-显性-上位性主基因+加性-显性多基因混合控制,主基因遗传率分别是37.11%、57.76%;在大田环境中,苗期抗性鉴定符合加性-显性-上位性多基因模型(C0),多基因遗传率为26.86%,而成株期鉴定属于2对主基因+多基因模型(E2),主基因遗传率为36.57%。研究表明,由于植物抗性基因的表达具有时空性,台烟8号对CMV的抗性遗传在不同的时间和环境具有一定的差异;但随着植株的生长,抗性遗传趋于稳定,在成株期时,2个不同的环境均表现为2对主基因+多基因控制,所以对烤烟CMV抗性品种选育和改良要以主基因为主,同时注重环境的影响。
The joint segregation analysis method of mixed major gene plus polygene genetic model was used to study the inheritance of CMV disease resistance in flue-cured tobacco.In two different environments,four generations（P1,P2,F1,and F2） from the crosses between Taiyan-8 which was highly resistant to CMV disease as the maternal parent and NC82 which was susceptible to CMV disease used as the paternal parent were investigated.It was found that CMV resistance genetic model fitted model E1 both at seeding stage and adult stage in green house.The CMV resistance genes were controlled by two additive-dominance-epistatic major genes and plus-dominant multiple genes,and the heritabilities of major genes were estimated to be 37.11% and 57.76%,respectively.While,the CMV disease resistance genetic model fitted the additive-dominance-epistatic model（C0） and the heritability of the polygene was 26.86% at seeding stage in field.However,CMV resistance genetic model was identified to fit model E2 and the heritability of major genes was estimated to be 36.57% at adult stage.The results indicated that inheritance of Taiyan-8 resistance showed slightly different in different environments and growth stages for the temporal and spatial expression of plant resistance genes.With the growth of tobacco,CMV resistant genes were controlled by two major genes and polygene in two different environments.So major-gene was a main factor in genetic improvement ofCMV disease resistance whereas environmental effect should also be taken into consideration.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2014年第6期1278-1286,共9页
Journal of Plant Genetic Resources
基金
公益性行业(农业)科研专项项目(201203091)
中国烟草总公司科技重大专项项目(110201301009(JY-09))
中国烟草总公司科技重点项目(110201002002)
关键词
烤烟
CMV抗性基因
主基因+多基因
遗传模型
遗传率
flue-cured tobacco
CMV resistance genes
major-gene plus polygene
genetic model
heritability
作者简介
第一作者研究方向为作物遗传育种。E—mail：chenxiaocui0924@163．com
通信作者：张志明，主要从事玉米分子生物学研究。E-mail：zhangzm@sina．com．cn
通信作者：杨爱国，主要从事烟草遗传育种研究。E-mail：yangaiguo@caas．cn
