摘要
以苹果菠萝的顶芽和吸芽为外植体,接种于MS＋6-BA3 mg·L^-1＋蔗糖30g·L^-1的培养基上,30 d后腋芽萌发;再将新芽切割,接种于MS＋6-BA2 mg· L^-1 ＋NAA0.05 mg· L^-1＋蔗糖30g·L^-1的培养基上,30 ～40 d后形成丛生芽,每30～40 d继代1次,可增殖3～5倍;把丛生芽分割成单株并接种于MS＋6-BA 1 mg·L^-1 ＋AD3 mg·L^-1＋蔗糖30 g·L^-1培养基上,进行壮苗培养,25 ～30 d后再转接种于1/2MS＋NAA 0.5 mg· L^-1＋香蕉汁30g· L^-1＋蔗糖30g· L^-1培养基上,30 d后可形成完整的根系;小植株移栽成活率可达95％.
The apical buds and suckers of pineapple cultivar ‘ Apple’ （Ananas comosus ‘ Apple’） were inoculated onto MS medium containing 6-BA 3 mg · L^-1 and 30 g · L^-1 sucrose for inducing shoots.The shoots were bourgeoned after cultured for 30 d.They were then transferred onto MS medium supplemented with 6-BA 2 mg · L^-1,NAA 0.05 mg· L^-1 and 30 g · L^-1 sucrose for inducing cluster buds,and the cluster buds were produced after 30-40 d of culture.The proliferation ratio of the cluster buds was about 3-5 per month.The cluster buds were cultured on MS medium containing 6-BA 1 mg · L^-1,AD 3 mg · L^-1 and 30 g · L^-1 sucrose for about 25-30 d to produce strong shoots.These shoots were transferred onto 1/2 MS rooting medium containing NAA 0.50 mg · L^-1,30 g · L^-1 banana juice and 30 g · L^-1 sucrose,and the roots were induced to strike after about 30 d of culture.The rooted plantlets had about 95% of survival rate when transferred onto the soil.
出处
《热带生物学报》
2014年第3期277-279,共3页
Journal of Tropical Biology
关键词
苹果菠萝
组织培养
快速繁殖
Ananas comosus ‘Apple’
tissue culture
rapid propagation
作者简介
昝丽梅（1976-），女，助理农艺师．E—mail：zanlimei555@163．com
