摘要
【目的】铁氧化酶是氧化铁锰鞘细菌类在污水处理中最重要的酶,筛选铁氧化酶高产菌株,研究酶学特性及研究该菌株对水中铁、锰的吸附性能。【方法】利用尿素培养基富集、CGY平板从3份不同水样中分离产铁氧化酶菌株:富集培养铁氧化酶产生菌;摇瓶复筛铁氧化酶产量较高的菌株,通过菌落形态、镜检、生理生化试验和16S rRNA基因序列遗传分析对目的菌株进行鉴定。【结果】分离筛选到一株高产铁氧化酶的菌株S9,被鉴定为鞘细菌球衣菌属浮游球衣菌(Sphaerotilus natans);该菌株对铁锰具有较强的吸附性,当吸附时间为4h时,菌株对水中铁的吸附量为29.02mg/g,吸附率为66.77%;当吸附时间为6h时,菌株对锰的吸附量为34.49mg/g,吸附率为70.68%;该菌株产铁氧化酶的最适温度和p H分别为30℃和7.5,Mg2+、Na+、K+离子对铁氧化酶活性有一定的激活作用,Cu2+对铁氧化酶影响不大,而Mn2+、Zn2+对铁氧化酶具有很强的抑制作用,Pb2+、Ag+对铁氧化酶活性有一定的抑制作用。【结论】浮游球衣菌S9是一株具有较高铁氧化性的菌株,在治理污水和给排水工程中的应用具有潜在的应用价值。
[ Objective] We screened and isolated Ferro-oxidase producing bacteria, for adsorbing iron and manganese. [Methods] The strains producing Ferro-oxidase were isolated from three samples of water. Ferro-oxidase producing strains were screened in shake flask culture, and identified according to morphological features, physiological and biochemical analysis as well as 16S rRNA gene sequence analysis. [ Results] We isolated a bacterium S9. The strain was identified as Sphaerotilus natans. This strain had strongest adsorption on iron and manganese among the strains we identified, with 29.02mg/g iron adsorption amount in water, and 66.77% adsorption rate for 4 hours＇ adsorption. When the adsorption time is 6 h, the adsorption amount of manganese was 34.49mg/g, and the adsorption rate was 70.68%. The optimum temperature and pH value of Ferro-oxidase were 30℃ and 7.5, respectively. Mg^2＋, Na ^＋ , K^＋ could activate Ferro- oxidase, whereas Cu^2＋ had little impact. While Mn^2＋ , Zn^2＋ could strongly inhibit Ferro-Oxidase, Pb^2＋ , Ag ^＋ had only modest inhibitory effect. [ Conclusion ] Strain $9 had a high Ferro-oxidase activity, and has application potential in sewage treatment.
出处
《微生物学报》
CAS
CSCD
北大核心
2014年第12期1500-1506,共7页
Acta Microbiologica Sinica
基金
福建师范大学福清分校省级重点学科建设基金(20110346)
福建省大学生创新创业训练项目基金(SCX-2013026)~~
关键词
鞘细菌
铁氧化酶
筛选
鉴定
sheathed bacteria, Ferro-oxidase, screening, sewage treatment
作者简介
郑虹（1981-），女，福建省福清人，硕士，实验师，主要从事微生物菌株筛选及发酵。E—mail：zhh148@163．com
Tel：＋86-591-85254164；E—mail：75447136@qq．com
