摘要
背景:胚胎发育潜能的评估将直接影响胚胎移植的选择和辅助生殖的临床结局。目的:对早期胚胎发育潜能评估的方法进行归纳分析。方法:由第一作者检索1990年1月至2013年12月PubMed数据库相关文献,检索词为"Assisted Reproductive Technology,ART;Pre-implantation Embryo;Embryonic development viability;Evaluation methods",文献语言限制在英文。根据纳入排除标准,最终纳入63条进行分析探讨。结果与结论:形态学评估方法由于其快速、无创、简单等特点而得到广泛的应用。但随着辅助生殖技术的发展,新的胚胎评估方法成为研究的热点,形态学评分系统与实时成像分析系统相结合对早期胚胎发育过程连续观察便于选择优胚。通过分析培养液中的代谢产物变化来预测胚胎发育潜能(丙酮酸、葡萄糖、氨基酸等)、胚胎源性细胞因子(可溶性人白细胞抗原G1、血小板活化因子等);针对基因、染色体异常而产生的植入前遗传学筛查法等以期找到更有效地评估胚胎发育潜能的标记物。
BACKGROUND：Embryo viability assessment is directly related to the selection of embryo transplantation and clinical outcome of assisted reproduction. OBJECTIVE：To summarize the assessment methods for early embryonic development. METHODS：The first author searched PubMed database for relevant articles published from January 1990 to December 2013 using the keywords of“assisted reproductive technology, art;pre-implantation embryo;embryonic development viability;evaluation methods”in English. Final y, 63 articles were included in result analysis. RESULTS AND CONCLUSION：In the embryo quality evaluation, the most widely used method is morphological evaluation method which is characterized as rapid, non-invasive, and simple. With the development of assisted reproductive technology, the morphological evaluation combined with time-lapse imaging analysis system has been recognized in embryo selection. Recently, targeted-metabolic analysis has been proposed as a useful tool for assessment of embryo development potential, involving pyruvate acid, glycometabolism, amino acid, and embryo-derived cytokines （soluble human leukocyte antigen G1, platelet-activating factor, etc.）. Furthermore, the pre-implantation genetic screening method targeting gene and chromosome abnormality is expected to find more effective markers for evaluating embryo developmental potential.
出处
《中国组织工程研究》
CAS
CSCD
2014年第42期6849-6855,共7页
Chinese Journal of Tissue Engineering Research
基金
广西卫生厅自筹经费科研项目(Z22010457)~~
作者简介
李楠，男，1984年生，河南省沁阳市人，汉族，2012年广西大学毕业，博士，助理研究员，主要从事胚胎工程研究。
通讯作者：韦继红，硕士，主任医师，广西柳州市妇幼保健院生殖健康助孕中心，广西壮族自治区柳州市545001
