摘要
目的：建立以HPLC法测定雏痢净超微粉中的苍术素含量的方法。方法：采用Thermo C18色谱柱（250mm×4.6mm,5μm）,甲醇-水（79∶21）为流动相,流速1.0mL·min^-1,检测波长为340nm。结果：苍术素在0.102μg·mL^-1~2.04μg·mL^-1范围内与峰面积呈良好的线性关系（R2=1.000 0）,精密度RSD=0.098 4%（n=6）,重复性RSD=1.168%（n=6）,供试品溶液在24 h内稳定性良好,平均加样回收率为99.20%（RSD=0.959%）。结论：该方法操作简便、准确、重现性好,可用于复方微粒的质量控制。
Objective： The method of HPLC with UV detection for the determination of atractylodin in the Chulijing superfine powder was established. Method： The chromatographic conditions included that： Thermo C18（250 mm×4.6 mm,5μm）was used as chromatographic column;the methanol- water（79∶21）as mobile phase;the flow rate was 1.0 m L/min,the detection wave length was 340 nm. Result： The calibration curves for atractylodin were linear in the range of 0.102 ~2.04ug·ml-1（R2=1.000 0）,RSD of instrument precision was 0.098 4%（n=6）,RSD was 1.168%（n=6）,samples＇ solution was stable within 24 h,and the average recovery was 99.20%（RSD =0.959%）.Conclusion： The method, which is easy and simple to handle with good repeatability,can be used for the quality control of the compound Chulijing superfine powder.
出处
《山西科技》
2014年第6期41-43,共3页
Shanxi Science and Technology
基金
山西省科技厅2011年科技攻关项目"兽药‘雏痢净’超微粉体关键技术研究"(项目编号:20110321081-02)
关键词
高效液相色谱
苍术素
雏痢净
超微粉
HPLC
atractylodin
Chinese medicine Chulijing
superfine powder
作者简介
李晓艳，女，1980年11月生，2004年毕业于山西中医学院中药学专业，工程师，山西省医药与生命科学研究院，山西省太原市，030006．
