摘要
目的探讨维生素D受体(VDR)基因FokⅠ和BsmⅠ位点多态性与北京地区汉族老年男性2型糖尿病脂代谢异常的相关性。方法采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)和基因测序技术,检测328例北京汉族老年男性VDR基因FokⅠ和BsmⅠ位点基因型和等位基因频率,同时与相关临床指标进行比较。其中2型糖尿病患者(DM组)237例与正常对照(NC组)91例。根据血脂情况将DM组分为非血脂异常组(DO组)134例和血脂异常组(DH组)103例。结果 VDR基因FokⅠ和BsmⅠ位点基因频率分布符合Hardy-Weinberg平衡,具有群体代表性。VDR基因FokⅠ位点F等位基因频率在DM组较NC组显著升高(χ2=3.873,P=0.049,OR=1.439,95%CI:1.001-2.071)。在显性模型下,DM组FF基因型频率较NC组显著升高(χ2=5.057,P=0.025,OR=1.756,95%CI:1.072-2.875);DH组FF基因型频率较NC组显著升高(χ2=6.168,P=0.013,OR=2.06,95%CI:1.161-3.663);其余各组组间比较,基因型及等位基因频率差异均无统计学意义(P>0.05);与Ff+ff基因型比较,FF基因型人群的平均舒张压水平显著降低,而餐后2 h血糖、甘油三酯、尿酸的水平显著升高,差异有统计学意义(分别是P=0.039;P=0.035;P=0.049;P=0.031)。BsmⅠ位点基因型及等位基因频率在各组间的差异均无统计学意义(P>0.05)。bb基因型人群血肌酐水平显著高于BB+Bb基因型,差异有统计学意义(P=0.011)。结论 VDR基因FokⅠ位点多态性可能是老年男性2型糖尿病合并脂代谢异常的危险因素,而BsmⅠ多态性可能与老年男性2型糖尿病无相关。
Objective To assess the association of vitamin D receptor（VDR） gene Fok I and Bsm I polymorphisms with dyslipidemia in elderly male patients with type 2 diabetes of Han nationality. Methods A total of 328 elderly male residents of Han nationality in Beijing, including 237 type 2 diabetic patients and 91 healthy control subjects, were enrolled in this study.The diabetic patients were divided into non-dyslipidemia group（DO group, n=134） and dyslipidemia group（DH group, n=103）. All the participants were genotyped for Fok I and Bsm I polymorphisms in VDR gene using polymerase chain reactionrestriction fragment length polymorphism（PCR-RFLP） and DNA sequencing technology, and the results were compared with their clinical characteristics. Results For Fok I, the frequency of F allele was significantly higher in the diabetic patients than in the control group（χ^2=3.873, P=0.049, OR=1.439, 95% CI： 1.001-2.071）. In the dominant model, the frequency of FF genotype was significantly higher in the diabetic group（χ^2=5.057, P=0.025, OR=1.756, 95% CI： 1.072-2.875） as well as in DH group（χ^2=6.168, P=0.013, OR=2.06, 95% CI： 1.161-3.663） than in the control group. There was no significant differences in the genotype frequency or allele distribution in other paired groups（P〉0.05）. Compared with Ff ＋ ff genotype, FF genotype was associated with a significantly decreased average diastolic blood pressure（P=0.039） but significantly increased postprandial blood glucose（P=0.035）, triglycerides（P=0.049） and uric acid（P=0.031）. No significant difference was detected in genotype frequency or allele distribution of Bsm I polymorphisms between the groups（P〉0.05）; serum creatinine levels were significantly higher in bb genotype than in BB ＋ Bb genotype group（P=0.011）. Conclusion VDR gene Fok I polymorphisms may be a risk factor for dyslipidemia in elderly male patients with type 2 diabetes among Chinese Han population, where Bsm I polymorphisms are not associated with diabetic dyslipdiemia.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2014年第11期1562-1568,共7页
Journal of Southern Medical University
基金
军事医学创新专项(13CXZ029)
关键词
维生素D受体
2型糖尿病
血脂异常
单核苷酸多态性
vitamin D receptor
type 2 diabetes mellitus
dyslipidemias
single nucleotide polymorphisms
作者简介
夏征，在读硕士研究生，E-mail：286162039@qq．com
张红红，研究员，硕士生导师，E-mail：zhanghh301@sina．com
