摘要
油菜素甾醇(BR)是一类重要的调控植物生长发育的激素.脱落酸(ABA)是一类重要的帮助植物适应逆境的激素.之前的研究表明BR和ABA信号对萌发率的调控存在拮抗作用,BR合成或信号缺失的突变体在萌发率上对ABA更加敏感.为了进一步研究BR与ABA信号通路互作的分子机制,采用化学诱变剂甲基磺酸乙酯(EMS)诱变bri1-301(BR受体BRI1的一个弱突变体),0.75μmol/L ABA的培养基上筛选对ABA不敏感的突变体.经过多次连续世代筛选,获得了大约40个稳定的株系,发现其中8个株系与abi1、abi5 ABA不敏感突变体的萌发率相似.通过利用PCR扩增ABI1、ABI2、ABI3、ABI4和ABI5基因并测序,鉴定到bri1-301背景下的4个ABA不敏感突变体abi2-8、hab2-1、abi3-21和abi4-13,表明ABA与BR信号的互作可以不依赖BR受体调控种子的萌发,为ABA与BR信号互作提供了遗传的证据.
Brassinosteroid（BR）,the plant steroid hormones,play important roles in regulating a wide range of plant growth and developmental processes.The plant hormone abscisic acids（ABA）play a key role in helping plants tolerate the stress environments.Previous studies showed that BR and ABA regulate the germination rate antagonistically.Plants deficient in brassinosteroid（BR）biosynthesis or defective in signal transduction are more sensitive to ABA in germination rate.To further study the molecular mechanism of crosstalk between BR and ABA signal pathway,we carried out Ethyl Methyl Sulfonate（EMS）screening on bri1-301,a weak mutant of BR receptor protein BRI1.Then we sowed the mutated seeds on medium with 0.75μmol/L ABA to select ABA insensitive candidates.Through repetitive screenings on continuous generations,we obtained about 40 stable ABA insensitive lines.Eight of the forty lines have the similar germination rates as ABA insensitive mutants abi1 and abi5,we amplified ABI1,ABI2,ABI3,ABI4 and ABI5 genes of these candidates by PCR and sequenced them.We identified four ABA insensitive mutants in bri1-301 background,abi2-8,hab2-1,abi3-21 and abi4-13,indicating the crosstalk between ABA and BR pathway does not depend on BR receptor to regulate seed germination,which provides genetic evidence for the crosstalk between ABA and BR.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2014年第4期550-556,566,F0003,共9页
Journal of Fudan University：Natural Science
基金
国家自然科学基金重大研究计划培育项目(90817004)
国家自然科学基金杰青项目(30925020)
上海市科技人才计划项目(11XD1400700)
作者简介
李泳池（1988-），女，硕士研究生
王学路，男，教授，通讯联系人，E-mail：xueluw@gmail．com．
