摘要
目的分析A群轮状病毒（RV）引起婴幼儿腹泻的感染情况。方法利用胶体金法A群轮状病毒试剂对本院2012年1月-2012年12月间,15 132例年龄〈6岁的腹泻患儿粪便轮状病毒抗原进行检测;对11月份进行了流动患儿和本地患儿的对比分析;对流动患儿和本地患儿的阳性标本随机各抽取10%,共126例样本,采用逆转录PCR（RTPCR）法进行轮状病毒血清G、P基因分型。结果 15 132例样本中阳性3 067例,阳性率20.27%;其中年龄≤6个月的患儿阳性率9.65%（435/4 510）;年龄6个月-2岁的患儿阳性率26.55%（2 377/8 953）;年龄2岁-6岁的患儿阳性率15.28%（255/1 669）;阳性率以10月、11月、12月、1月较高,其中11月最高,为53.71%;11月份流动患儿和本地患儿的对比分析,流动患儿阳性率58.50%（430/735）,以G3P[8]为主;本地患儿阳性率51.52%（829/1 609）,以G3P[8]为主。结论轮状病毒感染有其特殊性,在年龄上以6个月-2岁患儿感染率最高;还有明显的季节性,以秋冬季感染较高;流动患儿感染率高于本地患儿,且有相同的基因表现型。
Objective To analyze the infection of diarrhea in infants and children caused by the group A rotavirus（ RV）.Methods 15 132 children under 6 years old old with diarrhea rotavirus from January to December in 2012 were detected by colloidal gold reagent; comparing and analysis the different between the flowing and the local children suffering from RV; 10%of the 126 positive cases of samples were randomly selected and rotavirus serum G,P genotyping was conducted using reverse transcription PCR（ RT- PCR） method. Results There were 3067 positive cases among the 15 132 samples,the positive rate of which was 20. 27%; the positive rate was 9. 65%（ 435 /4 510）,26. 55%（ 2 377 /8 953）,15. 28%（ 255 /1 669） respectively when the children＇s age was under 6 months,6 months to 2 years old,2 years old to 6 years old; the positive rate was higher in October,November and December,the highest was in November; Comparing and analyzing the flowing and local children in November,thevpositive rate of flowing children was 58. 50%（ 430 /735）,based on G3P[8]; the positive rate of local children was 51. 52%（ 829 /1609）,based on G3P[8]. Conclusion The infection of rotavirus has its particularity,the highest infection rate was the children detected from the age of 6 months to 2 years old; there is obvious seasonal infections in autumn and winter; rate of the infection in flow children is higher than the local children,and have the same phenotype.
出处
《中国卫生检验杂志》
北大核心
2014年第19期2852-2854,共3页
Chinese Journal of Health Laboratory Technology
关键词
轮状病毒
阳性率
特殊性
基因分型
Rotavirus
Positive rate
Particularity
Genotyping
作者简介
赵成国（1977-），男，本科，主管技师，主要从事临床检验工作。
