摘要
自甘肃地区采摘的冬虫夏草子座和内核菌上经组织分离得到冬虫夏草菌,经形态学鉴定为中国被毛孢,利用该菌株进行液体发酵.结果表明:温度18℃、溶氧量160r/min、接种量10%、pH自然;优化配方为:葡萄糖30g/L、蚕蛹粉20g/L、KH2PO41.0g/L、MgSO4·7H2O 0.9g/L,2g/L(NH4)2SO4+0.1g/L VB1,发酵8天可得菌丝体产率为17.36g/L.
The pure culture spawn,obtained by tissue isolation from cordyceps sinensis picked in the mountainous areas of Gansu Province,was identified as Hirsutella sinensis by morphological observations.The yield of mycelium reached 17.36 g/L by optimizing liquid fermentation conditions.The results showed that the yield of mycelium was successively related to glucose,silkworm pupa protein powder （SPP）,KH2PO4,and MgSO4 · 7H2O.The optimized liquid fermentation conditions was as follows：temperature of 18 ℃,dissolved oxygen amount of 160 r/min,inoculation level of 10％,30 g/L glucose,20 g/L SPP,1.0 g/L KH2PO4,0.9 g/L MgSO4 · 7H2O,2 g/L （NH4）2SO4 and 0.1 g/L VB1.
出处
《甘肃科学学报》
2014年第5期20-23,共4页
Journal of Gansu Sciences
基金
甘肃省科学院应用研究与开发项目(2012JK-04)
甘肃省科学院青年科技创新基金项目(2014QN-07)
关键词
甘肃
冬虫夏草
中国被毛孢
液体发酵
Gansu
Cordyceps sinensis
Hirsutella sinensis
Liquid fermentation
作者简介
秦鹏（1984-）,男,甘肃天水人,硕士,助理工程师,主要从事食（药）用真菌的遗传育种及其种质资源利用开发和信息化技术研究.E-mail：kingkerberos@163.com
通讯作者：E—mail：wanglong-0106@163．com
