摘要
目的:研究环境雌激素(environmental estrogens,EEs)中壬基酚(NP)对体外培养子宫肌瘤细胞的作用效应及可能机制。方法:对子宫肌瘤细胞进行原代、传代培养及细胞鉴定。CCK-8法分析不同浓度NP在24小时、48小时、72小时对子宫肌瘤细胞活性的作用效应。采用荧光定量RT-PCR法检测各干预组雌激素受体α(ERα)mRNA、胰岛素样生长因子-1(IGF-1)mRNA、血管内皮生长因子(VEGF)mRNA的表达。采用Western Blot法检测ERα、IGF-1、VEGF蛋白的表达情况。结果:不同浓度的NP作用于子宫肌瘤细胞24小时、48小时、72小时,结果显示NP对子宫肌瘤细胞有促增殖作用,这种作用具有时间和浓度的依赖性。不同浓度的NP促进ERα、IGF-1、VEGF相关基因和蛋白的表达,该作用随着NP作用浓度的增加而增强,差异有统计学意义(P<0.05)。结论:NP可促进子宫肌瘤细胞增殖,其作用机制可能与通过ERα介导的IGF-1、VEGF等促肿瘤生长因子的表达增加有关。
Objective：To study the effect of environmental estrogens nonylphenol（NP）on uterine leiomyoma（UL）cells. Methods： Primary UL cells were cultured and treated with NP in various doses and durations （24,48 and 72 hrs）. The viability of cells were determined by CCK-8,and the expressions of ERa,IGF-1 and VEGF were detected by real-time quantitative PCR and western blot. Results：NP promoted the growth of UL cells and the expressions of ER（x,IGF-1 and VEGF mRNA and protein. The viability of cells treated with NP was time-and dose-dependent. Conclusions： NP could promote the growth of UL cells,which might be associated with the ER-rnediated activation of growth factors such as IGF-1 and VEGF.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2014年第9期686-690,共5页
Journal of Practical Obstetrics and Gynecology
基金
江苏省卫生厅青年科技基金项目(编号:Q201305)
南京市科技计划项目(编号:201201054)
关键词
子宫肌瘤
环境雌激素
壬基酚
雌激素受体Α
胰岛素样生长因子-1
血管内皮生长因子
Uterine leiomyoma
Environmental estrogen
Nonylphenol
Estrogen receptor-α
Insulin-likegrowth factor 1
Vascular endothelial growth factor
作者简介
通讯作者：沈杨，Email：shenyang0924@sina．cn
