摘要
目的 探讨妊娠期糖尿病（GDM）患者胎盘组织中胎球蛋白A（AHSG）基因mRNA及蛋白表达与胰岛素抵抗的关系.方法 采用反转录聚合酶链反应（RT-PCR）、Western blot技术检测43例正常足月妊娠剖宫产患者（对照组）和45例GDM患者（GDM组）胎盘组织中AHSG基因mRNA及蛋白表达水平,并检测剖宫产前空腹血糖（FPG）、空腹胰岛素（FINS）,计算稳态模型评估法胰岛素抵抗指数（HOMA-IR）.结果 GDM组FPG、FINS和HOMA-IR水平均显著高于对照组[（5.28±0.63） mmol/L比（4.24±0.21） mmol/L,（11.75±4.85） mU/L比（9.73±2.18） mU/L,2.79±1.02比1.87±0.66],差异有统计学意义（P＜0.01或＜0.05）;GDM组胎盘组织AHSG基因mRNA和蛋白表达水平显著高于对照组（0.7616±0.0691比0.3294±0.025 1,0.824 5±0.1375比0.420 3±0.075 0）,差异有统计学意义（P＜0.01或＜0.05）;GDM组HOMA-IR水平与胎盘组织AHSG基因mRNA和蛋白表达水平呈正相关（r=0.633,0.587,P＜ 0.05）.结论 GDM患者胎盘组织中AHSG基因mRNA和蛋白表达水平显著升高,胰岛素抵抗在GDM患者发病中有重要作用.
Objective To investigate the relationship between expressions of alpha 2-HS-glycoprotein （AHSG） gene mRNA and protein in placenta and insulin resistance in patients with gestational diabetes mellitus （GDM）.Methods The expressions of AHSG gene mRNA and protein in placenta were detected by reverse transcriptase-polymerase chain reaction （RT-PC R） and Western blot technique in normal uterogestation uterine-incision delivery patients （control group,43 cases） and patients with GDM （GDM group,45 cases）.Fasting plasma glucose （FPG） and fasting insuhn （FINS） were detected before uterine-incision delivery,and the homeostasis model of assessment for insulin resistance index （HOMA-IR） was computed.Results The FPG,FINS and HOMA-IR in GDM group were significantly higher than those in control group [（5.28 ± 0.63）mmol/L vs.（4.24 ±0.21） mmol/L,（11.75 ±4.85） mU/L vs.（9.73 ±2.18） mU/L,2.79 ±1.02 vs.1.87 ± 0.66],there were statistical differences （P 〈 0.01 or 〈 0.05）.The expressions of AHSG gene mRNA and protein in GDM group were significantly higher than those in control group （0.761 6 ± 0.069 1 vs.0.329 4 ± 0.025 1 and 0.824 5 ± 0.137 5 vs.0.420 3 ± 0.075 0）,there were statistical differences （P〈 0.01or 〈 0.05）.HOMA-IR level in GDM group was positively correlated with AHSG gene mRNA and protein （r =0.633,0.587,P 〈0.05）.Conclusions The expressions of AHSG gene mRNA and protein are significantly higher in patients with GDM.Insulin resistance may play an important role in the pathogenesis and development of GDM.
出处
《中国医师进修杂志》
2014年第27期7-9,共3页
Chinese Journal of Postgraduates of Medicine
基金
深圳市宝安区科技计划（2013282）
关键词
甲胎蛋白类
糖尿病
妊娠
胎盘
胰岛素抵抗
Alpha-fetoproteins
Diabetes,gestational
Placenta
Insulin resistance
作者简介
通信作者：曹聪明，Email：chizhiyiheng168@126．com
