摘要
目的观察糖痛方对糖尿病大鼠肢体缺血再灌注后血浆、肝、肾细胞间黏附分子-1(ICAM-1)和髓过氧化物酶(MPO)表达的影响,并探讨其保护作用机制。方法采用链脲佐菌素(STZ)糖尿病大鼠模型,饲养1个月后通过暂时阻断腹主动脉、髂总动脉和股动脉造成肢体缺血再灌注模型,随机分组后灌胃给药8周,检测各组大鼠血浆、肝、肾ICAM-1和MPO的表达情况。结果与正常组大鼠比较,模型组大鼠血浆、肝、肾ICAM-1和MPO表达水平明显升高,经糖痛方治疗后,ICAM-1和MPO表达水平明显下降。结论糖痛方能够降低糖尿病大鼠肢体缺血再灌注后血浆、肝、肾ICAM-1和MPO的表达水平。
Objective To observe the expression level of intercellular adhesion molecule-1 （ICAM-1） and myeloperoxidase （MPO） in plasma,liver and kidney of DRSIRIL treated by Tangtongfang,and to explore the protective mechanism.Methods Diabetic rats were induced by streptozotocin （STZ） and fed for one month.DRSIRIL model was maked by keeping abdominal aorta,common iliac artery and femoral artery blocked temporarily.Grouped randomly,administered intragastrically for eight weeks,the rats of different groups were killed to detect the expression of ICAM-1 and MPO in plasma,liver and kidney.Results Compared with normal rats,the expression levels of ICAM-1 and MPO in plasma,liver and kidney of DRSIRIL were obviously higher.The expression levels of ICAM-1 and MPO decreased significantly after DRSIRIL were treated by Tangtongfang.Conclusion Tangtongfang can reduce the expression level of ICAM-1 and MPO in plasma,liver and kidney of DRSIRIL.
出处
《医学研究杂志》
2014年第8期37-40,共4页
Journal of Medical Research
基金
国家自然科学基金资助项目(81173445)
作者简介
通讯作者：林兰，主任医师、博士生导师，电子信箱：linlan05@163．com
