摘要
目的研究叉头样转录因子3（Foxp3）的表达及启动子区甲基化水平在肝细胞性肝癌（HCC）与正常肝组织中的差异及其在HCC发病中的作用。方法使用荧光定量PCR检测39例HCC及13例正常肝组织中Foxp3mRNA水平，通过焦磷酸测序的方法检测其启动子区甲基化水平；组间比较采用秩和检验，相关性分析采用Spearman秩相关，P＜0．05为差异有统计学意义。结果Foxp3mRNA在肝癌组织中相对表达量为8．54±18．30，高于正常肝组织的4．88±14．72，两组比较，Z=-2．770，P=0．0056，差异有统计学意义。Foxp3启动子区A位点的甲基化水平在正常肝组织为90．60±3．50，高于肝癌组织的80．96±20．86，两组比较，Z=2．118，P=0．0339，差异有统计学意义；而正常肝组织和肝癌组织Foxp3启动子区其余B、C、D、E4个位点和整体甲基化水平比较，P值均＞0．05，差异均无统计学意义。Foxp3 mRNA水平与A位点的甲基化水平相关性分析r=-0．344，P=0．046，呈负相关；而其余4个位点与FoxD3 mRNA水平均无相关性∽值均〉0．05）。结论Foxp3高表达与Foxp3启动子区A位点低甲基化可能参与肝癌的发生和发展。
Objective To gain insights into the role of forkhead box protein 3 （Foxp3） in the pathogenesis of hepatocellular carcinoma （HCC） by performing a comparative analysis of Foxp3 mRNA expression and promoter methylation status in HCC and normal liver tissues. Methods Thirty-nine HCC and 13 normal liver tissue specimens were evaluated by real-time quantitative PCR and pyrosequencing to measure the expression of Foxp3 mRNA and determine the methylation status of its promoter, respectively. Statistical analyses of the data were conducted by rank-sum test and Spearman＇s rank correlation coefficient test. Results The HCC specimens showed significantly higher mRNA expression of Foxp3 （vs. normal liver tissues, Z = -2.770, P = 0.0056）. Moreover, the HCC specimens showed significant hypomethylation of the Foxp3 promoter site A （vs. normal liver tissues, Z = 2.118, P = 0.0339）, and the Foxp3 rnRNA level was negatively correlated with the methylation of site A （rs = -0.344, P = 0.046）. None of the other four sites in the Foxp3 promoter showed a significant difference in methylation, and the overall methylation was not significantly different between the HCC and normal liver tissues. Conclusion Overexpression and low methylation of Foxp3 may be involved in the oncogenic and nrogression processes of HCC.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2014年第8期616-619,共4页
Chinese Journal of Hepatology
基金
陕西省2012科技计划项目（NO.2012K13-01-14）
作者简介
党珊女，34岁，医学硕士。E-mail：shah．dang@gmail．com.
