摘要
目的:优选核桃楸叶总鞣质的大孔树脂纯化工艺。方法:利用静态吸附-洗脱试验考察AB-8,D101,NKA-9,Daion HP-20型大孔树脂的吸附与洗脱能力,通过单因素试验考察上样量、水洗用量及洗脱溶媒对核桃楸叶总鞣质纯化工艺的影响。采用干酪素法测定核桃楸叶总鞣质含量,检测波长760 nm。结果:选用AB-8型大孔吸附树脂,最佳纯化工艺为上样液质量浓度2.672 g·L-1,上样量250 mL,洗脱流速2 BV·h-1,加水6 BV洗脱除杂,收集50%乙醇洗脱液10 BV;总鞣质洗脱率、纯度分别为73.32%,35.02%。结论:AB-8型大孔树脂对核桃楸叶总鞣质的分离性能较好,优选的纯化工艺适用于工业化生产。
Objective：To optimize purification process of total tannins from Juglans mandshurica leaf by macroporous resin.Method：Adsorption and elution capacities of AB-8,D101,NKA-9,Daion HP-20 macroporous resin were investigated by static adsorption-elution tests,single factor tests were adopted to optimize purification process of total tannins from J.mandshurica leaf with sample volume,water amount,elution solvent as factors.The content of total tannins was measured by casein,detection wavelength was set at 760 nm.Result：AB-8 macroporous resin was selected,its optimal purification technology was as follows：the concentration of sample solution 2.672 g ·L-1,sample volume of 250 mL,elution rate of 2 BV ·h-1,washed impurity with 6 BV of water,collected 10 BV of 50％ ethanol eluent; elution rate and purity of total tannins were 73.32％ and 35.02％,respectively.Conclusion：AB-8 macroporous resin had goog separation performance for total tannin from J.mandshurica leaf,this optimized purification process was suitable for industrial production.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第16期36-38,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
黑龙江省科技攻关项目(GC12C110)
哈尔滨市科技创新人才研究专项(2012RFQYS042)
关键词
核桃楸叶
大孔吸附树脂
总鞣质
干酪素法
Juglans mandshurica leaf
macroporous resin
total tannins
casein law
作者简介
尚作华，硕士，从事中药化学成分分析研究，Tel：0451-55665478，E-mail：874048661@qq．com
[通讯作者]王伟明，博士，研究员，硕士生导师，从事中药及保健食品研发，Tel：0451-55665478，E-mail：zyyjy@163．com
