摘要
[目的]探讨低频电针干预早期神经痛对背根神经节(dorsal root ganglion,DRG)辣椒素受体(Transient receptor potential vanilloid type 1,TRPV1)磷酸化与痛敏相关神经肽降钙素基因相关肽(calcitonin gene-related peptide,CGRP)与P物质(substance P,SP)的抑制作用。[方法]SD大鼠随机分为正常组、假手术组、模型组与电针组,每组8只。模型组采用脊神经结扎(spinal nerve ligation,SNL)的方法制作大鼠神经痛模型。电针组采用2 Hz电针治疗,选取患侧"足三里"、"昆仑"穴,连续3d。检测D1、D3大鼠术侧后足缩腿阈(Paw withdrawal threshold,PWT),D3 L5 DRG p-TRPV1、CGRP、SP水平。[结果]SNL模型大鼠早期即出现自发性疼痛,PWT显著下降(P<0.001),L5 DRG p-TRPV1水平升高(P<0.001),CGRP水平升高(P<0.05),SP水平下降(P<0.05)。SNL假手术组大鼠PWT没有显著变化。2 Hz电针能提高SNL模型大鼠的PWT(P<0.001),降低L5 DRG p-TRPV1水平(P<0.05)与CGRP水平(P<0.01),对SP水平没有显著影响。[结论]早期神经痛与DRG p-TRPV1、CGRP水平升高有关。低频电针能下调DRG p-TRPV1与CGRP水平,改善早期神经痛。
[Objective] To investigate the inhibitive effects of electroacupuncture（EA） with low frequency on DRG p-TRPV1,CGRP and SP during early neuropathic pain.[Methods] SD rats were randomly divided into Normal,Sham spinal nerve ligation（SNL）,SNL,and SNL ＋EA groups,8 animals per group.Rat neuropathic pain model was made by right L5 SNL in SNL group.2 Hz EA was administered at ipsilateral points Zusanli and Kunlun for consecutive 3 days in SNL ＋EA group.D1 and D3 PWT,D3 L5 DRG TRPV1 phosphorylation（p-TRPV1）,CGRP and SP levels were measured.[Results] Rats in Sham SNL group had no obvious change in PWT,while rats in SNL group developed spontaneous pain and had a significant reduction in PWT（P〈0.001）.L5 DRG p-TRPV1 and CGRP levels were increased（P〈0.001,P〈0.05）,while SP level was reduced（P〈0.05） in SNL rats.2 Hz electroacupuncture up-regulated SNL rats PWTs（P〈0.001）,reduced L5 DRG p-TRPV1 and CGRP levels（P〈0.05,P〈0.01）,and had no significant effect on L5 DRG SP level.[Conclusion] Early neuropathic pain was associated with up-regulation of DRG p-TRPV1 and CGRP.Electroacupuncture with low frequency could alleviate early neuropathic pain through reducing DRG p-TRPV1 and CGRP levels.
出处
《浙江中医药大学学报》
CAS
2014年第6期780-784,共5页
Journal of Zhejiang Chinese Medical University
基金
国家自然科学基金资助项目(81072855
81303039)
浙江省自然科学基金资助项目(LY12H27015
Z2100979)
国家中医药管理局重点学科(针灸学)~~
作者简介
方剑乔，E-mail：fangjianqiao7532@163．com
