摘要
目的:禾谷炭疽菌侵染玉米、小麦等农作物引起的炭疽病,给各国农业生产造成了巨大经济损失。植物病原菌在对植物侵染、定殖、扩展等致病过程中,细胞信号转导起着重要的作用。磷脂酰肌醇转移蛋白(PITP)参与众多生理生化过程,并在细胞信号转导过程中发挥重要作用。本研究为深入开展禾谷炭疽菌PITP的功能研究打下坚实的理论基础,同时,也为进一步开展其他炭疽菌的研究提供重要的理论指导。方法:基于酿酒酵母中已经报道的5个典型PITP序列,对炭疽菌属蛋白质数据库进行Blastp比对以及关键词搜索,并通过SMART保守结构域分析。结果:明确禾谷炭疽菌存在3个典型的PITP,同时,明确上述蛋白疏水性、细胞信号肽、跨膜区结构、亚细胞定位以及二级结构等情况。结论:与其他物种中9个同源序列进行遗传关系比较分析,该菌与希金斯炭疽菌亲缘关系较近。
Objective： CoUetotrichum graminicola can infect corn, wheat and other food crops, causing tremendous economic losses in agricultural production in many countries. The cellular signal transduction plays an important role in the pathogenic process of plant pathogens infection, colonization, and expansion. PITP plays an important role in many physiological and biochemical processes. Which can provide strong theoretical foundation to the function of PITP, but also provide an important theoretical guidance to clarify the other pathogen in Colletotrichum spp.. Method：Based on the five typical PITP sequences have been reported in Saccharomyces cerevisiae, to search PITP related protein sequence from the protein databases of Colletotrichum spp. with the Blastp as well as the use of words. Meanwhile, bioinformatics analysis was made including the hydrophobic, signal peptide, trans - membrane domain structure, sub - cellular location and the secondary structure in three PITP proteins. Result：There are three typical PITPs in C. graminicola, and some characters were identified in this study. Conclusion：The results showed that C. graminicola close genetic relationship with C. higginsanum through comparative three PITPs in C. graminicola with other species of 9 homologous sequences.
出处
《生物技术》
CAS
CSCD
北大核心
2014年第3期39-43,共5页
Biotechnology
基金
云南省森林灾害预警与控制重点实验室开放基金项目("滇西地区核桃炭疽病菌的防治及多菌灵对其作用机制研究"
编号:ZK11A101
"滇西地区核桃炭疽病菌的鉴定及对多菌灵抗药性研究"
编号:ZK11SB01)资助
作者简介
作者简介：韩长志（1981-），男，博士，讲师，研究方向：经济林木病害生物防治与真菌分子生物学，发表论文lO余篇，Email：hanchangzhi@gmail．com。
