摘要
在现行PCR检测鸭坦布苏病毒(DTMUV)方法的基础上,设计制备DTMUV竞争模板并以其为定量内标物建立竞争定量PCR(QC-PCR)体系。该体系特异性强、灵敏性高,竞争模板和目标模板可在400个分子/μL的水平上共扩增。临床应用结果表明,建立的体系能够满足简单快速确定病毒含量的要求。
Based on the traditional PCR method for DTMUV detection , the competitive template targeting to DTMUV NS5 gene was designed and used as competitor in the quantitatively competitive PCR ( QC-PCR) system with high specificity and sensitivity .The target and competitive templates could be co -amplified at the level of 400 molecules per microliter .The clinical application results indicated that the developed QC -PCR could simply , rapidly and quantitatively diagnose the content of DTMUV .
出处
《山东农业科学》
2015年第3期113-117,共5页
Shandong Agricultural Sciences
基金
科技部科技基础性工作专项"创新方法在农业科技计划中的应用"
