摘要
用毛细管区带电泳 -电化学检测法同时测定甘露糖及其杂质葡萄糖和葡萄糖酸钙的含量 .研究了工作电极电位、电泳运行液浓度和酸度、分离电压及进样时间等对分离和检测的影响 .以直径为 3 0 0 μm的铜圆盘电极为工作电极 ,在工作电位为 0 .65 V(vs.SCE) ,运行缓冲液为 60 mmol/L Na OH的条件下 ,3种组分在1 6min内完全分离 .甘露糖、葡萄糖和葡萄糖酸钙在 2 .0～ 0 .0 1 mmol/L范围内均成线性 ,检测下限分别为6.1、7.1和 3 .7μmol/L.相对标准偏差分别为 3 .6%、2 .4%和 3 .8% ,加标回收率 (n=3 )分别为 1 0 0 .6%、1 0 0 .1 %和 98.9% .
A simultaneous determination of mannose, glucose and calcium gluconate formed in mannose production by using capillary zone electrophoresis combined with electrochemical detection(CE ED) is reported. The effects of working electrode potential, running buffer concentration, separation voltage and injection time on the determination were examined. Mannose, glucose and calcium gluconate could be well separated in a 60 mmol/L NaOH buffer within 16 min. A 300 μm diameter copper disk electrode was used as a working electrode at a potential of 0 65 V( vs .SCE). A good linear relationship between peak current and analyte concentration in the range of 2 0~ 0 01 mmol/L was obtained with detection limit of 6 1 μmol/L?7 1 μmol/L and 3 7 μmol/L for mannose , glucose and calcium gluconate, respectively. The RSD of the peak currents for the analytes were 3 6%, 2 4% and 3 8%( n =7), respectively. Recoveries for mannose, glucose and calcium gluconate were 100 6%, 100 1%, and 98 9%( n =3) respectively.
出处
《应用化学》
CAS
CSCD
北大核心
2002年第7期629-632,共4页
Chinese Journal of Applied Chemistry
基金
中国科学院长春应用化学研究所电分析化学开放实验室资助课题
