摘要
目的 :探讨钌红对严重烧伤早期心肌细胞和线粒体损害的治疗作用。方法 :健康成年Wistar大鼠 2 4只 ,随机分为正常对照组、烧伤组和钌红治疗组 (n =8)。烧伤组、钌红治疗组大鼠造成 30 %TBSAⅢ°烧伤 ,伤后30min经腹腔补液。钌红治疗组同时于颈外静脉推注钌红 (剂量 2mg/kg) ,3h后再推注 1次。烧伤组和钌红治疗组动物于伤后 6h抽血并活杀。测定线粒体呼吸功能、Ca2 + 浓度 ([Ca2 + ]m)及血清LDH、CK。结果 :钌红治疗组较烧伤组 [Ca2 + ]m显著降低 ,线粒体呼吸控制率 (RCR)、Ⅲ态呼吸速率 (ST3)明显升高 ,Ⅳ态呼吸速率(ST4 )降低 ,钌红治疗组血清CK、LDH显著低于烧伤组 ,分别是烧伤组的 65 .0 %和 45 .5 %。结论
Objective:To investigate the effects of ruthenium red on myocyte and mitochondrial damage during the early stage after severe burn.Methods:Wistar rats were randomized into normal control group (Control), burns group (Burns) and ruthenium red treated group (RR) (n=8).Rats of Burns and RR were scalded to 30% TBSA Ⅲ 0, and 30 minutes later, resuscitated with Ringer's solution. Ruthenium red was injected to rats of RR in dose of 2mg/kg, and again 3 hours later.After rats were sacrificed at the 6th hour postscalding,myocardial mitochondrial respiratory function and Ca 2+ concentration ([Ca 2+ ]m),serumal CK and LDH were determined.Results: [Ca 2+ ]m, ST 4,CK and LDH of RR were significantly lower than those of Burns.However,mitochondrial respiratory control rate (RCR) and ST 3 were higher than those of Burns.Conclusion:Ruthenium red attenuated myocyte and mitochondrial damage during the early stage after severe burn.
出处
《中国现代医学杂志》
CAS
CSCD
2002年第10期4-6,共3页
China Journal of Modern Medicine
基金
国家重点基础研究发展规划项目基金资助 (编号G19990 54 2 0 2 )
