摘要
目的 比较三种促进组织工程化人工骨体内血管化方法的效果及研究血管化与成骨的相关关系。 方法 将HA/β TCP与PDLLA形成复合支架材料 ,再复合Ⅰ型胶原、rhBMP 2 ,与成骨细胞联合培养 ,仿生制备成组织工程化人工骨。采用包裹带血管蒂筋膜、复合血管内皮细胞或两者联合的促血管化手段 ,将人工骨修复兔桡骨干骨膜 骨完全缺损。手术后 4、8、12周 ,观察移植物组织学 ,体视学方法观察血管化、成骨作用及其两者的关系。 结果 3种方法均有促血管作用 ,其优劣依次为 :材料包裹带血管蒂筋膜及复合血管内皮细胞大于材料单纯复合血管内皮细胞 ,后者又大于材料单纯包裹带血管蒂筋膜。术后 4周为快速血管化阶段 ,4～ 8周间血管化有平缓发展 ,12周完全血管化。血管化与新骨生成数量成正相关。 结论 促血管化手段对组织工程化人工骨移植修复骨缺损的效果起重要促进作用。
Objective To compare the effects of three various methods to reinforce vascularization of tissue engineered bone transplanted to long bone defect and investigate the relationship between vascularization and osteogenesis Methods The scaffold materials of tissue engineered bone were composed of HA/β TCP and PDLLA coated with type Ⅰ collagen and rhBMP 2 The osteoblasts were cocultured with above material to construct tissue engineered bone Pedicled fascia, coculture of endothelial cells combined these two methods were applied to promote vascularization The tissue engineered bone then was transplanted to repair total segmental periosteum bone defect The osteogenesis, vascularization and their relationship were observed after 4,8,12 weeks of operation Results The three methods all had effect of vascularization. The sequence of superiorit was: pedicled fascia in addition of coculture of endothelial cells>coculture of endothelial cells>pedicled fascia The first 4 weeks was the period of fast vascularization which was relatively slowly reinforced during the next four weeks Complete vascularization appeared after 12 weeks of operation There was a positive correlation between osteogenesis and vascularization Conclusion The approaches to reinforce vascularization of tissue engineered bone can greatly improve the repair results especially in the segmental defect of long bone
出处
《中华显微外科杂志》
CSCD
北大核心
2002年第2期119-122,共4页
Chinese Journal of Microsurgery
基金
国科科技部国家重点基础研究发展规划项目 (973)资助课题 (G1 9990 5430 8)
国家自然科学基金重点资助项目 (39830 1 0 0 )
关键词
组织工程化人工骨
血管化
研究
骨缺损
修复
Tissue engineered bone
Vascularization
Bone defect
Osteogenesis
