摘要
探讨胚胎期汗腺发生过程中表皮生长因子(EGF)、基质金属蛋白酶 2 (MMP 2 )和MMP 7与汗腺发生的相关性 ,为诱导表皮干细胞向汗腺细胞定向分化奠定基础。分别取 13～31周胚龄人胎儿背部全层皮肤 ,以免疫组织化学染色法 (SP法 )动态观察汗腺胚芽细胞或汗腺细胞及其周围局部基质对基质金属蛋白酶MMP 2、MMP 7与EGF及汗腺胚芽细胞或汗腺细胞对细胞角蛋白 7(K7)蛋白质表达情况 ,并以原位杂交法动态观察汗腺胚芽细胞或汗腺细胞MMP 2、MMP 7的mRNA信号特征。结果显示 ,MMP 2、MMP 7、EGF在14～2 0周于汗腺细胞及其周围局部基质蛋白质表达逐渐加强 ,2 0～ 2 2周达峰值 ,并维持至所观察的全时段 ;MMP 2、MMP 7的mRNA信号强弱在汗腺胚芽细胞或汗腺细胞中与其蛋白质表达相吻合 ;K7始于 14～ 16周在汗腺芽细胞内表达 ,并持续存在。研究表明 ,汗腺于胚龄 14～ 16周开始发生 ,至第 2 4周基本成熟 ;汗腺发生与MMPs所致细胞外基质改建。
The development of sweat glands is a very complex biological process, which involves many factors. In this study, the correlation between epidermal growth factor (EGF), matrix metalloproteinases (MMP 2,MMP 7) and development of sweat glands in human embryos was explored. Furthermore, the mechanism(s) underlying the induction of epidermal stem cells into sweat gland cells was elucidated. Skin biospies of human embryos obtained from spontaneous abortions at different gestational ages from 11 to 31 weeks were used. The dynamical expression of EGF, MMP 2, MMP 7 and keratin 7(K7) in developing sweat gland cells or extracellular stroma surrounding the sweat gland cells were examined with S P immunohistochemical methods. The localization of the cellular sources of MMP 2 and MMP 7 was examined with in situ hybridization. The results showed that at 14 20 wk of gestation, a gradual increase of EGF immunoreactivity was observed not only in developing sweat gland buds but also in extracellular stroma surrounding the buds,and the expression intensity peaked at 20～22 wk of gestational age. All mRNA positive buds or cells in developing sweat glands contained corresponding immunoreactive proteins. The immunostaining for K7 appeared in early sweat gland buds at 14～16wk of gestation, and from then on, K7 was concentrated in developing sweat gland cords or cells. It is suggested that the morphogenesis of sweat gland in human fetal skin begins at 14～16wk of gestational age, and essentially completes by 24wk. There is a close relationship among EGF ,extracellular matrix remodeling and morphogenesis of sweat glands, and EGF is one of the inducers in the development and maturity of sweat gland buds or cells.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第5期383-385,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家重大基础研究规划资助项目 (编号G1 9990 542 0 4 )
国家自然科学基金 (编号 30 1 70 966)
国家杰出青年科学基金 (编号 3952 50 2 4 )
关键词
肝腺发生
表皮生长因子
基质金属蛋白酶
烧伤
动物实验
morphogenesis sweat glands
embryo
epidermal growth factor
matrix metalloproteinases
