摘要
目的 了解陕西地区乙肝病毒 (HBV)基因分型情况 .方法 用套式 PCR方法从 10 0份 HBs Ag阳性的乙型肝炎患者血清中扩增 5 85 bp的 S基因 ,用限制性内切酶 Bsr ,Sty ,Dpn 和 H pa 平行酶切鉴别 HBV A～ F基因型 .结果 所有标本用内引物 P1和 P2均扩增获得的 5 85 bp的靶基因 ,继之用上述限制性内切酶消化鉴定 ,证明 10 0份血清标本中的 HBV可分为 B型 36份 (36 % ) ,C型 5 6份 (5 6 % ) ,D型 8份 (8% ) .结论 陕西地区 HBV分型以 B,C型为主 ,D型较少 ,未发现 A,E,F型 .结果同时表明 PCR- RFL P方法灵敏性高 ,特异性强 ,且酶切图谱简明单一 ,易于识别 。
AIM To investigate the genotypes of Hepatitis B virus (HBV) in Shaanxi province. METHODS The 585 bp length of HBV S gene was amplified from the blood samples of 100 patients with HBsAg positive using polymerase chain reaction (PCR). The PCR products were digested with four kinds of restriction enzymes, Bsr Ⅰ, Sty Ⅰ, Dpn Ⅰ and Hpa Ⅱ to determine the genotyping of HBV. RESULTS By PCR RFLP, HBV from patients with hepatitis B can be divided into three types, i.e. type B, C and D, and the percentages of type B, type C and type D were 36%, 56% and 8% respectively. CONCLUSION Genotype of HBV in Shaanxi mainly consists of type B and type C, and type D is rare in this region.
出处
《第四军医大学学报》
北大核心
2002年第8期746-748,共3页
Journal of the Fourth Military Medical University
