摘要
目的 观察体外蒿甲醚与血红素伍用对日本血吸虫磷酸甘油酸激酶（PGK）和丙酮酸激酶（PK）的影响。方法 以 4～5周龄的血吸虫,置于含蒿甲醚和/或血红素的培素液内培养24h后,测定虫体的PGK和PK酶活力。结果体外,蒿甲醚（50μmol/L）或血红素（50μmol/L）对血吸虫的PGK和PK酶活力无影响,但两者伍用可引起两种酶的活力明显下降。结论 蒿甲醚对血吸虫PGK和PK的影响具有血红素依赖性。
ve To study the effect of combining artemether with heme on activities of phosphoglyc-erate(PGK) and pyruvate kinase(PK) in adult Schistosoma japonicum in vitro. Methods The activities oj PGK and PK were measured in paired worms incubated without or with artemether and/ or heme for 24 h in vitro. Results No effect on the activities of PGK and PK was observed in the worms exposed to artemether(50μmol/L)or heme(50μmol/L) alone for 24h. However, the combined chemotherapy led to a significant decrease in the PGK and PK activities in the parasite. Conclusion Artemether showed a heme - dependent effect on the PGK and PK activities in schistosomes.
出处
《中国热带医学》
CAS
2002年第1期9-11,共3页
China Tropical Medicine
