摘要
目的 建立体外大量扩增猪树突状细胞 (Dendriticcells ,DC)的方法 ,并进行形态学和免疫学性质鉴定。方法 制备猪骨髓细胞 ,分离单个核细胞 ,以猪重组GM CSF诱导培养 ,2周后收集悬浮细胞 ,进行形态学观察和免疫细胞表面分子鉴定 ,以及功能检测。结果 体外培养富集的猪DC达 70 %～ 80 % ,具有典型的形态、表型及功能。结论 成功地建立了体外大量扩增猪骨髓DC的方法 。
Objective To amplify dendritic cells(DC) from porcine bone marrow and study their morphological,immunological characteristics. Methods Lymphocytes in porcine bone marrow were isolated on a Ficoll separation gradient. The selected cells were cultured with granulocyte macrophage colony stimulating factor(GM CSF), and the suspensive cells were collected 2 weeks after cultivation. The morphological features and numbers of DC were identified and counted with light microscopy. The co stimulation molecules on membrane surface were studied by FACS. The function was observed by means of MLR. Results The percentage of DC was 70%～80% in the DC enriched populations as confirmed under microscope. The cells showed typical morphology of DC and expressed high level of B7 molecules and were potent stimulators of human T cells in MLR. Conclusion The method to generate large numbers of DCs from porcine bone marrow is established, which might contribute to further study on the role of DC in xenotransplantation and tolerance induction.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2002年第3期255-257,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助重大项目 ( 399934 30 2 )
