摘要
为了探讨尿液pH值与产后泌乳奶牛瘤胃酸中毒的关系及其预警作用,试验随机选取产后90 d内荷斯坦奶牛40头,依据健康奶牛尿液pH值范围(在7.7～8.7之间)将其分为酸性组(pH值<7.7)和中性组(pH值在7.7～8.7之间),通过Afikim管理软件查询试验牛瘤胃酸中毒情况,测定试验牛尿液pH值,并对试验牛血液/尿液中的矿物质元素、炎性因子、能量代谢指标的水平进行组间独立样本t检验,利用斯皮尔曼相关系数分析相关性,二元回归分析预测疾病,最后利用工作特征曲线(ROC)分析确定分界值。结果表明:通过Afikim管理软件查询,酸性组奶牛20 d内瘤胃酸中毒发病率显著高于中性组(P<0.05),酸性组与中性组相比尿液pH值显著降低(P<0.05);酸性组与中性组相比,矿物质元素血液K含量显著降低(P<0.05)、尿液Ca和P含量显著升高(P<0.05),炎性因子脂多糖(LPS)含量显著升高(P<0.05),能量代谢指标β-羟丁酸(BHBA)、血尿素氮(BUN)、尿液尿素氮(UUN)和游离脂肪酸(NEFA)含量显著升高(P<0.05),葡萄糖(GLU)含量显著降低(P<0.05);相关性分析显示,瘤胃酸中毒与尿液pH值呈极显著负相关(P<0.01),与尿液Ca、P呈极显著正相关(P<0.01),与血液NEFA呈显著正相关(P<0.05);二元回归分析显示,尿液pH值、P和血液Ca、NEFA这4项营养代谢指标可作为奶牛瘤胃酸中毒的发生的风险预测指标;ROC分析确定,瘤胃酸中毒的最佳尿液pH值的分界值<6.55,尿液pH值能作为奶牛瘤胃酸中毒的预警指标。说明产后泌乳奶牛尿液pH值可作为预测奶牛瘤胃酸中毒发生风险的评估指标。
In order to investigate the relationship between urine pH value and rumen acidosis in postpartum lactating cows and its early warning effect,the experiment randomly selected 40 Holstein cows within 90 days after delivery,and then divided them into an acidic group ( pH value<7. 7) and a neutral group ( pH value between 7. 7- 8. 7) according to the urine pH range of healthy cows ( between 7. 7- 8. 7) . The Afikim management software is used to query the rumen acidosis of the test cattle,and the pH value of the test cow urine is determined. The levels of mineral elements,inflammatory factors and energy metabolism indicators in the blood /urine of the test animals were tested by independent sample t test between groups,using Spearman correlation coefficient to analyze correlation,binary regression analysis to predict disease, and finally using working characteristics curve ( ROC) analysis to determine the cutoff value. The results showed that the incidence of ruminal acidosis in the acidic group was significantly higher than that of the neutral group within 20 days of the Afikim management software ( P<0. 05) . The pH value of the acidic group was significantly lower than that of the neutral group ( P< 0. 05) . Compared with the neutral group,the blood K content of mineral elements of the acidic group decreased significantly ( P<0. 05) ,the urine Ca and P levels of the acidic group increased significantly ( P<0. 05) ,and the inflammatory factor tallow lipopolysaccharide ( LPS) content of the acidic group increased significantly ( P< 0. 05) ,energy metabolism indicators β - hydroxybutyric acid ( BHBA) ,blood urea nitrogen ( BUN) ,urine urea nitrogen ( UUN) and free fatty acid ( NEFA) content of the acidic group increased significantly ( P<0. 05) ,glucose ( GLU) content of the acidic group decreased significantly ( P<0. 05) . Correlation analysis showed that ruminal acidosis was negatively correlated with urine pH value significantly ( P<0. 01) ,and it was positively correlated with urine Ca and P significantly ( P<0. 01) . There was a significant positive correlation between blood NEFA ( P<0. 05) . Binary regression analysis showed that urine pH value,P and blood Ca and NEFA could be used as risk predictors for rumen acidosis in dairy cows.ROC analysis determined that the cut off value of the optimal urine pH value for rumen acidosis was<6. 55,urine pH value can be used as an early warning indicator for rumen acidosis in dairy cows. It indicates that the urine pH value of postpartum lactating cows can be used as an indicator to predict the risk of rumen acidosis in dairy cows.
作者
孙光野
白云龙
夏成
徐闯
张洪友
SUN Guangye;BAI Yunlong;XIA Cheng;XU Chuang;ZHANG Hongyou(Collge of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2019年第3期76-79,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
国家重点研发计划项目(2017YFD0502200)
黑龙江省应用技术研究与开发计划重大项目(GA16B202)
作者简介
孙光野(1992-) ,男,硕士研究生,研究方向为临床兽医学,940495242@ qq.com;通信作者: 张洪友(1960-) ,男,教授,硕士,硕士生导师,研究方向为动物营养代谢病,zhy478@ 163.com.
