摘要
目的 探讨ADP-核糖基化因子6抑制剂对脓毒症致急性肾损伤模型的保护作用。 方法 2018年2月将30只雄性BALB/c小鼠按随机数表法分为未感染组(5只)、氟康唑组(5只)、ADP-核糖基化因子6抑制剂组(10只)(简称抑制剂组)和生理盐水对照组(10只)(简称对照组)。氟康唑组、抑制剂组和对照组小鼠经尾静脉注射1×10^5 CFU白色念珠菌建立脓毒症致急性肾损伤模型,未感染组注射等量生理盐水。3 h后抑制剂组注射1.032 mg ADP-核糖基化因子6抑制剂,氟康唑组注射51 μg氟康唑,对照组注射与抑制剂组等体积生理盐水。处理后24 h根据体征及症状对小鼠进行脓毒症严重程度临床评分,用Jaffe检测法及酶偶联速率法测定小鼠血清肌酐及尿素氮,用HE染色法制作病理切片后,根据每张切片中肾皮质组织损伤的面积对肾组织进行病理评分。 结果 未感染组临床评分、血清肌酐、尿素氮和病理评分分别为0分、(0.98±0.38)μmol/L、(9.77±0.36)mmol/L、(0.88±0.30)分,氟康唑组分别为(0.80±0.84)分、(1.09±0.51)μmol/L、(9.64±0.17)mmol/L、(1.22±0.270)分,抑制剂组分别为(2.8±1.32)分、(1.43±0.50)μmol/L、(12.05±1.20)mmol/L、(2.04±0.55)分,对照组分别为(5.20±1.87)分、(2.96±1.55)μmol/L、(13.94±1.94)mmol/L、(2.67±0.55)分。未感染组与对照组比较,血清肌酐和尿素氮差异有统计学意义(P<0.05),说明造模成功;抑制剂组与对照组各项指标相比差异均有统计学意义(P<0.05)。 结论 ADP-核糖基化因子6抑制剂能减轻小鼠真菌感染脓毒症致急性肾损伤的程度。
Objective To investigate the protective effect of ADP-ribosylation factor 6 inhibitor on acute kidney injury induced by sepsis in mice.Methods In February 2018, thirty male BALB/c mice were divided into uninfected group (5 mice), fluconazole group (5 mice), ADP-ribosylation factor 6 inhibitor group (10 mice)(inhibitor group) and saline control group (10 mice)(control group) by random number table method. In fluconazole group, inhibitor group and control group, 1×105 CFU of Candida albicans was injected via tail vein for modeling. The uninfected group was injected with equal volume of saline. After 3 hours, inhibitor group was injected with 1.032 mg ADP-ribosylation factor 6 inhibitor, and fluconazole group was injected with 51 μg fluconazole. The control group were injected with equal volume of saline as inhibitor group. After 24hours, serum creatinine, urea nitrogen were measured by kit method. The mice were clinically scored for sepsis severity according to signs and symptoms after treatment and histopathological changing of kidney tissue were observed and scored according to the damage area of renal cortical with hematoxylin-eosin staining.Results The clinical scores, serum creatinine, urea nitrogen and pathological scores of uninfected group were 0, (0.98±0.38)μmol/L, (9.77±0.36)mmol/L, (0.88±0.30), respectively. The fluconazole group were (0.80±0.84), (1.09±0.51)μmol/L, (9.64±0.17)mmol/L, (1.22±0.270), respectively. The inhibitor group were (2.80±1.32), (1.43±0.50) μmol/L, (12.05±1.20) mmol/L, (2.04±0.55), respectively). The control group were (5.20±1.87), (2.96±1.55) μmol/L, (13.94±1.94) mmol/L, (2.67±0.55). The difference was statistically significant between inhibitor group and the control group both (P<0.05).Conclusions ADP-ribosylation factor 6 inhibitor reduce acute kidney injury induced by sepsis in mice.
作者
沈思魁
黄燚
贾文文
冯师健
李虹
Shen Sikui;Huang Yi;Jia Wenwen;Feng Shijian;Li Hong(Department of Urology,West China Hospital,Sichuan University,Chengdu 610041,China;Department of Clinical Laboratory,Sichuan Academy of Medical Sciences & Siehuan Provincial Hospital,Chengdu 610072, China;Department of Ophthalmology,Siehuan Academy of Medical Sciences & Siehuan Provincial Hospital, Chengdu 610072,China)
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2019年第1期57-61,共5页
Chinese Journal of Urology
作者简介
通信作者:李虹,Email:wzwzlwww@qq.com.
