摘要
为探讨红沙枇杷与白沙枇杷冷藏耐储性差异的原因,为枇杷采后生理和保鲜技术研究提供参考,以白沙枇杷"白玉"和红沙枇杷"鸡蛋红"为材料,在6℃的冷藏条件下,测定了果皮中氧自由基产生速率(oxygenfree radical production rate, SPR)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等保护酶活性,以及膜脂过氧化伤害产物(MDA)的含量等活性氧(reactive oxygen species, ROS)代谢相关指标的变化规律。表明,随着冷藏的进程,冷藏的前10 d,红沙枇杷果皮SPR高于白沙枇杷,10 d后红沙枇杷果皮SPR有所下降而白沙枇杷果皮则大幅上升,导致10 d后红沙枇杷果皮SPR低于白沙枇杷;冷藏后红沙枇杷果皮ROS相关酶(SOD, POD和CAT)活性高于白沙枇杷果皮,即其ROS清除能力高于白沙枇杷果皮;在冷藏5 d后白沙枇杷果皮膜脂过氧化伤害产物MDA含量持续上升,而红沙枇杷果皮则维持在较低水平并低于白沙枇杷果皮,说明白沙枇杷果皮膜脂过氧化程度较高。分析认为冷藏初期红沙枇杷果皮较高的氧自由基产生速率激活了ROS清除系统,导致红沙枇杷果皮具有更高的ROS清除能力;冷藏后期白沙枇杷果皮则出现了氧自由基和MDA的积累,暗示其膜脂过氧化的发生和果实内外环境的恶化。红沙枇杷和白沙枇杷果皮ROS代谢的差异与冷藏耐储性相关。
To reveal the factors causing the differences between white and red flesh loquat storability during cold storage, and to provide a reference for postharvest physiology and preservation technology of loquat fruits, the white flesh loquat(cv. Baiyu) and red flesh loquat(cv. Jidanhong) were used as materials, the ROS-related indices of superoxide production rate(SPR), the protective enzymes activity of Superoxide dismutase(SOD), peroxidase(POD) catalase(CAT) and the fruit pericarp reactive oxygen species(ROS) metabolism indexes between them determined. were determined under 6℃ cold storage. The results have showed that SPR before the first ten days of cold storage was higher in the white flesh loquat pericarp than that in the red one. While the red flesh loquat pericarp SPR declined slightly and the white one rose greatly, which resulted in the higher SPR in the red one than that in the white one after 10 d time point. Higher pericarp antioxidant enzyme activities of SOD, POD and CAT inthe red flesh loquat pericarp than in the white one were observed after 5 d time point in cold storage conditions,which means the higher ROS-scavenging capability of the red flesh loquat pericarp than the white one in them.MDA content was constantly increasing in the white flesh loquat pericarp while the content remained at a relatively low level in the red one after 5 d time point in cold storage conditions. This indicated a higher membrane lipid peroxidation degree in the white flesh loquat pericarp than in the red one. It was concluded that the high oxygen free radical production rate production in the red flesh loquat pericarp at the early stage of cold storage activated ROS scavenging system, and which led to higher ROS scavenging ability in the red flesh loquat pericarp than in the white one. The accumulation of oxygen free radical production and MDA content in the white flesh loquat pericarp in the later stage of cold storage indicated a high degree of membrane lipid peroxidation and the environmental deterioration of extra and intracellular environment of the fruits. It could conclude that the metabolism differences correlated with storability of white and red flesh loquat pericarp during cold storage.
作者
刘恒蔚
颜婕
袁卫明
蒋梅
邱烨
顾晖
刘凯
Liu Hengwei;Yan Jie;Yuan Weiming;Jiang Mei;Qiu Ye;Gu Hui;Liu Kai(School of Chemistry,Biology and Materials Engineering,Suzhou University of Science and Technology,Suzhou,215009;Suzhou Polytechnic Institute of Agriculture,Suzhou,215008)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2019年第2期766-771,共6页
Genomics and Applied Biology
基金
苏州市科技计划项目(SYN201512)资助
关键词
枇杷
果皮
活性氧代谢
Loquat
Pericarp
Reactive oxygen metabolism
作者简介
通信作者:刘恒蔚,liuhw@mail.usts.edu.cn;通信作者:袁卫明,ywm357@163.com.
